A multicolor immunosensor for the visual detection of six sulfonamides based on manganese dioxide nanosheet-mediated etching of gold nanobipyramids.
A multicolor immunosensor for the visual detection of six sulfonamides based on manganese dioxide nanosheet-mediated etching of gold nanobipyramids.
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DOI：
10.1016/j.talanta.2023.124449
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发表时间：
2023-03
期刊：
影响因子：
6.1
通讯作者：
Qian Chen;Yuquan Pan;Chao-Yang Sun;Zongwen Wang;Yongning Wu;Fengfu Fu
中科院分区：
文献类型：
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作者：
Qian Chen;Yuquan Pan;Chao-Yang Sun;Zongwen Wang;Yongning Wu;Fengfu Fu
在实际生产中，为避免磺胺类药物产生耐药性，多种磺胺类药物在畜牧业中交替使用。因此，建立简便、高通量的检测方法，实现食品中水杨酸类农药残留的快速检测，具有重要意义。我们在此开发了一种灵敏有效的二氧化锰纳米片介导的金纳米双吡啶（AuNBPs）蚀刻，其可以产生更生动的颜色变化，并进一步制备了用于视觉筛选/半定量检测6种不同SA的高通量免疫传感器，包括磺胺二甲嘧啶（SMZ），磺胺甲氧嘧啶（SMD），磺胺嘧啶（SIM），磺胺甲基嘧啶（SMR），磺胺间甲氧嘧啶（SMM）和磺胺喹喔啉（SQ），通过使用AuNBP作为信号和广泛特异性抗SA抗体作为生物受体。该免疫传感器显示更生动的颜色变化，具有较低的视觉检测限和良好的特异性。该方法肉眼检测SMZ、SMD、SMR最低可达1.0 ng/mL，SIM、SMM、SQ最低可达2.0 ng/mL，紫外-可见分光光度法检测上述6种水杨酸最低可达0.2 ng/mL。该免疫传感器的视觉检测限远低于可食用组织中总水杨酸的最大残留限量（100 μg/kg）。该免疫传感器用于牛奶中SMZ、SMD、SIM、SMR、SMM和SQ的检测，回收率为84%~ 106%，相对标准偏差（n = 5）<8%。本研究的成功为食品中SMZ、SMD、SIM、SMR、SMM和SQ的现场快速检测提供了一种有前途的方法。重要的是，免疫传感器可以扩展为通过使用相应的宽特异性抗体作为生物受体来视觉筛选/半定量检测其他抗生素组的通用方法。
In reality, various sulfonamides (SAs) were alternately used in animal husbandry to avoid generating drug resistance. Thus, it is crucial to develop simple and high-throughput methods for detecting multiple or groups of SAs to realize rapid screening of total SAs residues in foods. We herein developed a sensitive and efficient MnO2nanosheets-mediated etching of gold nanobipyramids (AuNBPs), which can generate more vivid color changes, and further fabricated a high-throughput multicolor immunosensor for the visual screening/semi-quantitative detection of 6 different SAs including sulfamethazine (SMZ), sulfamethoxydiazine (SMD), sulfisomidine (SIM), sulfamerazine (SMR), sulfamonomethoxine (SMM) and sulfaquinoxaline (SQ) by using AuNBPs as signal and broad-specificity anti-SAs antibody as a bio-receptor. The immunosensor displays more vivid color changes, and has a lower visual detection limit and excellent specificity. It can be applied to detect as little as 1.0 ng/mL of SMZ, SMD, SMR and 2.0 ng/mL of SIM, SMM, SQ by bare eye observation, and 0.2 ng/mL of above 6 SAs by UV–visible spectrophotometry. The visual detection limit of the immunosensor is much lower than the maximum residue limit of total SAs (100 μg/kg) in edible tissues. The immunosensor was successfully applied to detect SMZ, SMD, SIM, SMR, SMM and SQ in milk with a recovery of 84%–106% and a RSD (n = 5) < 8%. The success of this study provided a promising assay for the on-site rapid screening of SMZ, SMD, SIM, SMR, SMM and SQ in food by bare eye observation. Importantly, the immunosensor may be expended as a general method for the visual screening/semi-quantitative detection of the group of other antibiotics by using the corresponding broad-specificity antibody as a bio-receptor.