Inhibition of metastasis-associated lung adenocarcinoma transcript 1 in CaSki human cervical cancer cells suppresses cell proliferation and invasion
Inhibition of metastasis-associated lung adenocarcinoma transcript 1 in CaSki human cervical cancer cells suppresses cell proliferation and invasion
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DOI：
10.1093/abbs/gmq008
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发表时间：
2010-03-01
影响因子：
3.7
通讯作者：
Li, Guancheng
中科院分区：
文献类型：
--
作者：
Guo, Fengjie;Li, Yalin;Li, Guancheng
转移相关肺腺癌转录本1（MALAT 1）被认为是一个长的（类似于7 kb）非编码RNA。MALAT 1在许多人类癌症中过表达，但其功能仍然未知。为了研究MALAT 1在人宫颈癌进展中的作用，我们设计并使用短发夹RNA抑制CaSki细胞中的MALAT 1表达，并验证其对细胞增殖和侵袭的影响。通过逆转录-聚合酶链反应分析基因表达的变化。我们的数据表明，MALAT 1通过调控caspase-3、caspase-8、Bax、Bcl-2和Bcl-xL等基因表达参与宫颈癌细胞的生长、细胞周期进程和侵袭，表明MALAT 1可能在宫颈癌生物学中具有重要意义。我们的研究结果说明了MALAT 1在宫颈癌进展中的生物学意义，并提供了新的证据表明MALAT 1可能作为预防人类宫颈癌的治疗靶点。
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is suggested to be a long (similar to 7 kb) non-coding RNA. MALAT1 is overexpressed in many human carcinomas, but its function remains unknown. To investigate the role of MALAT1 in human cervical cancer progression, we designed and used short hairpin RNA to inhibit MALAT1 expression in CaSki cells and validated its effect on cell proliferation and invasion. Changes in gene expression were analyzed by reverse transcriptase-polymerase chain reaction. Our data demonstrated that MALAT1 was involved in cervical cancer cell growth, cell cycle progression, and invasion through the regulation of gene expression, such as caspase-3, -8, Bax, Bcl-2, and Bcl-xL, suggesting that MALAT1 could have important implications in cervical cancer biology. Our findings illustrate the biological significance of MALAT1 in cervical cancer progression and provide novel evidence that MALAT1 may serve as a therapeutic target in the prevention of human cervical cancer.