Expression, purification, crystallization and preliminary X-ray analysis of a truncated soluble domain of human glioma pathogenesis-related protein 1.
Expression, purification, crystallization and preliminary X-ray analysis of a truncated soluble domain of human glioma pathogenesis-related protein 1.
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人胶质瘤发病机制相关蛋白1截短可溶结构域的表达、纯化、结晶和初步X射线分析。
DOI：
10.1107/s1744309110035669
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Asojo,OluwatoyinA
中科院分区：
文献类型：
--
作者：
Bonafe,Nathalie;Zhan,Bin;Bottazzi,MariaElena;Perez,OrianaA;Koski,RaymondA;Asojo,OluwatoyinA
胶质瘤发病相关蛋白1（GLIPR 1）是CAP超家族的成员，该家族包括来自广泛真核生物的蛋白质。包括GLIPR 1在内的大多数CAP蛋白的生物学功能尚不清楚。GLIPR 1在侵袭性胶质母细胞瘤中上调，并有助于培养的胶质母细胞瘤细胞的侵袭性。相反，GLIPR 1表达降低与晚期前列腺癌相关。强制性GLIPR 1过表达在前列腺癌细胞中是促凋亡的，并且正在临床试验中作为实验性前列腺癌治疗进行测试。将人GLIPR 1表达为截短的可溶性蛋白（sGLIPR 1），纯化并结晶。有用的X-射线数据已收集到超过1.9纳米分辨率的晶体，属于斜方晶系空间群P21212，平均晶胞参数a = 85.1，B = 79.5，c = 38.9纳米，单体或二聚体的不对称单元。
Glioma pathogenesis-related protein 1 (GLIPR1) is a member of the CAP superfamily that includes proteins from a wide range of eukaryotic organisms. The biological functions of most CAP proteins, including GLIPR1, are unclear. GLIPR1 is up-regulated in aggressive glioblastomas and contributes to the invasiveness of cultured glioblastoma cells. In contrast, decreased GLIPR1 expression is associated with advanced prostate cancer. Forced GLIPR1 overexpression is pro-apoptotic in prostate cancer cells and is being tested in clinical trials as an experimental prostate-cancer therapy. Human GLIPR1 was expressed as a truncated soluble protein (sGLIPR1), purified and crystallized. Useful X-ray data have been collected to beyond 1.9 Å resolution from a crystal that belonged to the orthorhombic space group P21212 with average unit-cell parameters a = 85.1, b = 79.5, c = 38.9 Å and either a monomer or dimer in the asymmetric unit.