Effect of annexin‐1 on experimental autoimmune encephalomyelitis (EAE) in the rat
Effect of annexin‐1 on experimental autoimmune encephalomyelitis (EAE) in the rat
复制标题
DOI：
10.1046/j.1365-2249.1998.00490.x
复制
发表时间：
1998-01
影响因子：
4.6
通讯作者：
Huitinga;Bauer;Strijbos;Rothwell;Dijkstra;Tilders
中科院分区：
文献类型：
--
作者：
Huitinga;Bauer;Strijbos;Rothwell;Dijkstra;Tilders
膜联蛋白1是一种钙依赖性磷脂结合蛋白，已被证明是一种内源性中枢神经保护剂，特别是对脑缺血损伤。在本研究中，我们将这些发现扩展到多发性硬化症EAE的动物模型中，并报道内源性膜联蛋白- 1在中枢神经系统（CNS）病变内的ED1+巨噬细胞和常驻星形胶质细胞中表达。在临床症状出现后，脑室内（icv）给予横跨膜联蛋白1 - 188氨基酸的NH2末端片段，可显著降低轻度EAE的神经系统严重程度和体重减轻。内源性脑膜联蛋白- 1的免疫中和并未加重EAE的临床特征。因此，尽管内源性膜联蛋白- 1在EAE发病机制中的作用仍有待确定，但我们的研究结果表明，膜联蛋白- 1可能对多发性硬化症的治疗有益。
Annexin‐1, a calcium‐dependent phospholipid binding protein, has been shown to act as an endogenous central neuroprotectant, notably against cerebral ischaemic damage. In the present study we extend these findings to an animal model of multiple sclerosis, EAE, and report that endogenous annexin‐1 is expressed in ED1+ macrophages and resident astrocytes localized within the lesions in the central nervous system (CNS). Intracerebroventricular (icv) administration of an NH2‐terminal fragment spanning amino acids 1–188 of annexin‐1 after the onset of the clinical symptoms significantly reduced both the neurological severity as well as weight loss of mild EAE. Immunoneutralization of endogenous brain annexin‐1 failed to exacerbate the clinical features of EAE. Thus, although the role of endogenous annexin‐1 in the pathogenesis of EAE remains to be determined, our findings suggest that annexin‐1 may be of therapeutic benefit to the treatment of multiple sclerosis.