Immune cell infiltration and growth-associated protein 43 expression correlate with pain in chronic pancreatitis
Immune cell infiltration and growth-associated protein 43 expression correlate with pain in chronic pancreatitis
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DOI：
10.1016/s0016-5085(97)70047-7
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发表时间：
1997-05-01
期刊：
影响因子：
29.4
通讯作者：
Buchler, MW
中科院分区：
文献类型：
--
作者：
DiSebastiano, P;Fink, T;Buchler, MW
背景与目的：慢性胰腺炎（CP）的神经支配模式和神经肽含量发生变化，包括生长相关蛋白43 （GAP-43）的神经元表达增加。方法：对29例CP患者的组织样本，采用数字化形态测量法测定其实质纤维化比、神经周围免疫细胞浸润程度、神经元GAP-43免疫反应性，并与个体疼痛评分进行相关性分析。结果：CP患者胰腺神经纤维和内在神经元中GAP-43明显升高。免疫细胞对胰腺神经的浸润程度与疼痛程度显著相关。疼痛评分与胰腺纤维化程度和病程均无相关性。结论：免疫细胞浸润胰腺神经和神经元可塑性是疼痛产生的致病因素，而胰腺纤维化程度对CP疼痛无主要影响。
Background & Aims: Changes in innervation pattern and neuropeptide content have been shown in chronic pancreatitis (CP), including increased neuronal expression of growth-associated protein 43 (GAP-43). We used GAP-43 as an established marker of neuronal plasticity and correlated histological findings with pain scores of patients with CP, Methods: In tissue samples from 29 patients with CP, the parenchyma-fibrosis ratio, degree of perineural immune cell infiltration, and neuronal GAP-43 immunoreactivity were determined by digitized morphometry and correlated with individual pain scores, Results: In CP, GAP-43 was significantly increased in pancreatic nerve fibers and intrinsic neurons. GAP-43 expression correlated with individual pain scores, The infiltration of pancreatic nerves by immune cells was significantly correlated with the intensity of pain. Pain scores correlated neither with the degree of pancreatic fibrosis nor with the duration of the disease, Conclusions: The results suggest that infiltration of pancreatic nerves by immune cells and neuronal plasticity are pathogenic factors for the generation of pain, whereas the degree of pancreatic fibrosis has no major impact on pain in CP.