Heterogeneous copper concentrations in cancerous human prostate tissues
Heterogeneous copper concentrations in cancerous human prostate tissues
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DOI：
10.1002/pros.23022
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发表时间：
2015-10-01
期刊：
影响因子：
2.8
通讯作者：
Cater, Michael A.
中科院分区：
文献类型：
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作者：
Denoyer, Delphine;Clatworthy, Sharnel A. S.;Cater, Michael A.
背景以铜为靶点治疗前列腺癌的疗法正在人体临床试验中进行评估。细胞内铜升高被认为会使前列腺癌细胞对某些铜配位化合物敏感，特别是那些具有离子传递特性的化合物。虽然有令人信服的体外证据表明前列腺癌细胞积累细胞内铜，但患者组织中铜的相应状态尚未得到证实。因此，我们确定了在整个疾病进展过程中患者的癌性前列腺组织和血清中的铜浓度是否增加。方法从患者前列腺切除术中获得人类前列腺组织样本（n = 28），并与患者匹配的血清一起通过电感耦合等离子体质谱法分析铜含量。结果当分组在一起时，表现出中度疾病严重程度（格里森评分7）（n = 10）的癌性前列腺组织中的铜含量是正常前列腺癌组织的1.6倍。年龄匹配的正常组织 (n=10) (P
BackgroundTherapeutics that target copper for the treatment of prostate cancer are being evaluated in human clinical trials. Elevated intracellular copper is considered to sensitize prostate cancer cells to certain copper-coordination compounds, especially those with ionophoric properties. While there is compelling in vitro evidence that prostate cancer cells accumulate intracellular copper, a corresponding status for copper in patient tissues has not been corroborated. We therefore established whether copper concentrations increase in cancerous prostate tissues, and in sera, in patients throughout disease progression.MethodsHuman prostate tissue samples were obtained from patient prostatectomies (n=28), and together with patient-matched sera, were analyzed for copper content by inductively coupled plasma mass spectrometry.ResultsWhen grouped together, cancerous prostate tissues exhibiting moderate disease severity (Gleason Score 7) (n=10) had 1.6-fold more copper than age-matched normal tissues (n=10) (P