Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity
Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity
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DOI：
10.3390/molecules24244450
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发表时间：
2019-12-01
期刊：
影响因子：
4.6
通讯作者：
Shim, Sang Hee
中科院分区：
文献类型：
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作者：
Lee, Changyeol;Li, Wei;Shim, Sang Hee
真菌菌株 Alternaria alternata JS0515 是从 Vitex rotundifolia（海滩牡荆）中分离出来的。分离出 12 种次生代谢物，包括一种新的阿藤菌素衍生物 (1)。分离的代谢物包括七种已知的阿藤菌素衍生物 (2-8)、两种异色满酮 (9, 10)、一种苝醌 (11) 和一种苯并环烷酮 (12)。通过一维和二维核磁共振 (NMR) 光谱、质谱 (MS) 和计算电子圆二色性 (ECD) 分析确定了它们的结构。化合物 3 和 11 增加 AD-293 人胚胎肾细胞中的丙酮酸脱氢酶 (PDH) 活性，并显着抑制 PDH 磷酸化。 3和11的IC50值分别为32.58和27.82μM。
The fungal strain Alternaria alternata JS0515 was isolated from Vitex rotundifolia (beach vitex). Twelve secondary metabolites, including one new altenusin derivative (1), were isolated. The isolated metabolites included seven known altenusin derivatives (2-8), two isochromanones (9, 10), one perylenequinone (11), and one benzocycloalkanone (12). Their structures were determined via 1D and 2D nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), and computational electronic circular dichroism (ECD) analysis. Compounds 3 and 11 increased pyruvate dehydrogenase (PDH) activity in AD-293 human embryonic kidney cells and significantly inhibited PDH phosphorylation. The IC50 values of 3 and 11 were 32.58 and 27.82 mu M, respectively.