THE ROLE OF PERIVASCULAR ADIPOSE TISSUE AND ENDOGENOUS HYDROGEN SULFIDE IN VASOACTIVE RESPONSES OF ISOLATED MESENTERIC ARTERIES IN NORMOTENSIVE AND SPONTANEOUSLY HYPERTENSIVE RATS
THE ROLE OF PERIVASCULAR ADIPOSE TISSUE AND ENDOGENOUS HYDROGEN SULFIDE IN VASOACTIVE RESPONSES OF ISOLATED MESENTERIC ARTERIES IN NORMOTENSIVE AND SPONTANEOUSLY HYPERTENSIVE RATS
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DOI：
10.26402/jpp.2019.2.13
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发表时间：
2019-04-01
影响因子：
2.2
通讯作者：
Berenyiova, A.
中科院分区：
文献类型：
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作者：
Cacanyiova, S.;Majzunova, M.;Berenyiova, A.
血管周围脂肪组织（PVAT）和硫化氢（H2S）在血管活性反应的调节中发挥重要作用，并且可以干扰原发性高血压的发病机制。本研究的目的是评估成年正常血压 (Wistar) 和自发性高血压大鼠 (SHR) 离体肠系膜动脉 (MA) 血管活性反应中 PVAT 和 H2S（内源性、外源性）之间的相互关系。在 SHR 中，高血压与心脏肥大和收缩力增加有关；然而，菌株之间的腹膜后脂肪量没有差异。 PVAT 揭示了两种菌株中外源性去甲肾上腺素诱导的血管收缩的抗收缩作用，但令人惊讶的是，这种作用在 SHR 中更强。同时；在 SHR 中，PVAT 对动脉交感神经释放的内源性去甲肾上腺素的收缩表现出促收缩作用，但在 Wistar 大鼠中则不然。我们证实了 H2S 对 Wistar 大鼠血管壁和 PVAT 的抗收缩作用，因为用 H2S 产生酶抑制剂炔丙甘氨酸 (PPG) 进行预处理，显着增加了去甲肾上腺素诱导的收缩。在 SHR 中，血管壁中的 H2S 表现出促收缩作用，但 PVAT 的存在消除了这种作用；然而，PPG预处理并没有进一步影响去甲肾上腺素收缩。然而，与 Wistar 大鼠不同，PVAT 的存在增强了 SHR 中外源施加 H2S 的血管舒张作用。我们的结果证实，MA 的 PVAT 和内源产生的 H2S 可以表现为促收缩或反收缩。在 SHR 中，与 Wistar 大鼠不同，PVAT 与血管周围神经刺激相关的促收缩作用以及 H2S 在动脉壁中的促收缩作用可以代表病理特征。另一方面，SHR的PVAT具有代偿性血管活性机制，包括未知因子（H2S以外）更强的抗收缩作用以及外源性H2S的血管舒张作用的增强。
Perivascular adipose tissue (PVAT) and hydrogen sulfide (H2S) play important roles in the modulation of vasoactive responses and can interfere with the ethiopathogenesis of essential hypertension. The aim of this study was to evaluate the mutual relationship between PVAT and H2S (endogenously produced, exogenous) in vasoactive responses of isolated mesenteric arteries (MA) in adult normotensive (Wistar) and spontaneously hypertensive rats (SHR). In SHR, hypertension was associated with cardiac hypertrophy and increased contractility; however, there were no differences in the amount of retroperitoneal fat between strains. PVAT revealed the anti-contractile effect on vasoconstriction induced by exogenous noradrenaline in both strains, but surprisingly, this effect was stronger in SHR. Concurrently; PVAT exhibited a procontractile effect on contractions to endogenous noradrenaline released from arterial sympathetic nerves in SHR, but not in Wistar rats. We confirmed the anti-contractile effect of H2S in both, the vascular wall and PVAT of Wistar rats because the pre-treatment with propargylglycine (PPG), an inhibitor of H2S producing enzyme, significantly increased the noradrenaline-induced contraction. In SHR, H2S in the vascular wall exhibited a pro-contractile effect that was eliminated by the presence of PVAT; however, the pre-treatment with PPG did not affect noradrenaline contraction farther. Nevertheless, unlike in Wistar rats, the presence of PVAT potentiated the vasorelaxant effect of exogenously applied H2S in SHR. Our results confirmed that PVAT of MA and endogenously produced H2S could manifest as pro-contractile or as anti-contractile. In SHR, unlike in Wistar rats, the pro-contractile effect of PVAT associated with the stimulation of perivascular nerves, and the pro-contractile effect of H2S in the arterial wall could represent pathologic features. On the other hand, PVAT of SHR is endowed with compensatory vasoactive mechanisms, which include stronger anti-contractile action of an unknown factor (other than H2S) and potentiation of the vasorelaxant effect of exogenous H2S.