Integrated shope virus DNA is present and transcribed in the transplantable rabbit tumour Vx-7.
Integrated shope virus DNA is present and transcribed in the transplantable rabbit tumour Vx-7.
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整合的 Shope 病毒 DNA 存在于可移植兔肿瘤 Vx-7 中并进行转录。
DOI：
10.1099/0022-1317-60-2-271
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Ito,Y
中科院分区：
文献类型：
--
作者：
McVay,P;Fretz,M;Wettstein,F;Stevens,J;Ito,Y
为了便于对Shope乳头状瘤病毒诱导的肿瘤细胞进行分子分析，我们已经建立了一个来自Vx-7的细胞系，Vx-7是一种最初由Shope病毒诱导的可移植肿瘤。采用单相分子杂交和Southern转移方法来评估DNA的拷贝数和物理状态以及转录的程度。发现肿瘤和细胞系都含有病毒基因组的多个拷贝，这些拷贝都整合到宿主细胞DNA中。转录对应的复杂性约。1%的病毒基因组以低丰度检出。这些结果进行了讨论，相对于我们早期的研究结果与肿瘤直接诱导的病毒，并要求维持VX-7肿瘤超过30年，它已经存在。
To facilitate a molecular analysis of Shope papilloma virus-induced neoplastic cells, we have established a cell line from Vx-7, a transplantable tumour originally induced by the Shope virus. Single phase molecular hybridization and Southern transfer methods were employed to assess copy number and physical state of the DNA, and the extent of transcription. Both tumour and cell line were found to contain multiple copies of the virus genome and these were all integrated into the host cell DNA. Transcripts corresponding to a complexity of approx. 1% of the virus genome were detected at low abundance. These results are discussed relative to our earlier findings with tumours induced directly by virus, and to requirements for maintenance of the Vx-7 tumour over the 30 years that it has been in existence.