Ultrasound and microbubble induced release from intracellular compartments.
Ultrasound and microbubble induced release from intracellular compartments.
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DOI：
10.1186/s12896-017-0364-3
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发表时间：
2017-05-18
影响因子：
3.5
通讯作者：
Karshafian R
中科院分区：
文献类型：
--
作者：
Hussein F;Antonescu C;Karshafian R
超声波和微泡 (USMB) 已被证明可以增强细胞内分子的摄取，通常认为这是声孔作用的结果。与 USMB 增强的细胞内摄取相关的潜在机制（例如膜破坏和内吞作用）也可能与 USMB 诱导的细胞物质释放到细胞外环境有关。本研究调查了 USMB 通过膜破坏和胞吐作用对细胞分子释放的影响。 USMB 诱导活细胞和非活细胞的细胞质中分别释放 19% 和 67% 的 GFP。经过 USMB 处理后，活细胞中早期/再循环内体释放的 Tfn 增加了 23%。此外，LAMP-1 抗体的 MFI 在活细胞中增加了 50%，表明 USMB 刺激了溶酶体胞吐作用。在非存活细胞中，在没有去垢剂细胞透化作用的情况下，LAMP-1 细胞内结构的标记表明 USMB 诱导的细胞死亡与溶酶体透化作用相关。总之，USMB 增强了细胞质、溶酶体和早期/再循环内体的分子释放。本文的在线版本 (doi:10.1186/s12896-017-0364-3) 包含补充材料，可供授权用户使用。
Ultrasound and microbubbles (USMB) have been shown to enhance the intracellular uptake of molecules, generally thought to occur as a result of sonoporation. The underlying mechanism associated with USMB-enhanced intracellular uptake such as membrane disruption and endocytosis may also be associated with USMB-induced release of cellular materials to the extracellular milieu. This study investigates USMB effects on the molecular release from cells through membrane-disruption and exocytosis. USMB induced the release of 19% and 67% of GFP from the cytoplasm in viable and non-viable cells, respectively. Tfn release from early/recycling endosomes increased by 23% in viable cells upon USMB treatment. In addition, the MFI of LAMP-1 antibody increased by 50% in viable cells, suggesting USMB-stimulated lysosome exocytosis. In non-viable cells, labeling of LAMP-1 intracellular structures in the absence of cell permeabilization by detergents suggests that USMB-induced cell death correlates with lysosomal permeabilization. In conclusion, USMB enhanced the molecular release from the cytoplasm, lysosomes, and early/recycling endosomes. The online version of this article (doi:10.1186/s12896-017-0364-3) contains supplementary material, which is available to authorized users.