Tracing activity across the whole brain neural network with optogenetic functional magnetic resonance imaging.
Tracing activity across the whole brain neural network with optogenetic functional magnetic resonance imaging.
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DOI：
10.3389/fninf.2011.00021
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发表时间：
2011
影响因子：
3.5
通讯作者：
Lee JH
中科院分区：
文献类型：
--
作者：
Lee JH
尽管有着压倒性的需求，但我们在追踪整个大脑网络水平活动的能力方面存在着相对较大的差距。大脑中复杂而密集的线路使得理解细胞类型的特定活动及其在几个突触之外的通信极具挑战性。光遗传学功能磁共振成像（ofMRI）的最新发展为脑回路的研究提供了新的动力，其能够因果追踪由整个脑中的限定细胞类型和放电模式引起的活动。脑回路元件可以基于它们的遗传身份、细胞体位置和/或它们的轴突投射目标以时间精度被选择性地触发，同时以前所未有的空间和时间精度非侵入性地监测所产生的网络响应。随着进一步的研究，包括技术创新，使ofMRI充分发挥其潜力，ofMRI预计将发挥重要作用，在我们的系统水平上了解大脑回路机制。
Despite the overwhelming need, there has been a relatively large gap in our ability to trace network level activity across the brain. The complex dense wiring of the brain makes it extremely challenging to understand cell-type specific activity and their communication beyond a few synapses. Recent development of the optogenetic functional magnetic resonance imaging (ofMRI) provides a new impetus for the study of brain circuits by enabling causal tracing of activities arising from defined cell types and firing patterns across the whole brain. Brain circuit elements can be selectively triggered based on their genetic identity, cell body location, and/or their axonal projection target with temporal precision while the resulting network response is monitored non-invasively with unprecedented spatial and temporal accuracy. With further studies including technological innovations to bring ofMRI to its full potential, ofMRI is expected to play an important role in our system-level understanding of the brain circuit mechanism.