A novel fluorescent probe diphenyl-1-pyrenylphosphine to follow lipid peroxidation in cell membranes
A novel fluorescent probe diphenyl-1-pyrenylphosphine to follow lipid peroxidation in cell membranes
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DOI：
10.1016/s0014-5793(00)01587-8
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发表时间：
2000-06-02
期刊：
影响因子：
3.5
通讯作者：
Noguchi, N
中科院分区：
文献类型：
--
作者：
Okimoto, Y;Watanabe, A;Noguchi, N
研究了二苯基-1-芘基膦（DPPP）作为荧光探针监测细胞膜脂质过氧化反应的可行性。DPPP与有机过氧化氢和过氧化氢按化学计量比反应生成DPPP氧化物（DPPP = O），DPPP掺入磷脂酰胆碱脂质体膜和多形核白细胞（PMN）与亚油酸甲酯过氧化氢迅速反应，但不与过氧化氢和叔丁基过氧化氢反应。这种新方法揭示了脂质过氧化在用佛波醇12-肉豆蔻酸酯13-乙酸酯刺激的PMNs的膜内进行，已知佛波醇12-肉豆蔻酸酯13-乙酸酯产生几种自由基。
Diphenyl-1-pyrrenylphosphine (DPPP) was tested whether it could be used as a fluorescent probe to monitor lipid peroxidation in cell membranes. DPPP reacted with organic hydroperoxides and hydrogen peroxide stoichiometrically to give DPPP oxide (DPPP = O), DPPP incorporated into phosphatidylcholine liposomal membranes and polymorphonuclear leukocytes (PMNs) reacted with methyl linoleate hydroperoxide rapidly but not with hydrogen peroxide nor wi:th tert-butyl hydroperoxide. This novel method revealed that lipid peroxidation proceeded within membranes of PMNs stimulated with phorbol 12-myristate 13-acetate, which is known to produce several, kinds of free radicals, It was found that DPPP is a suitable fluorescent probe to monitor lipid peroxidation within cell membranes specifically, (C) 2000 Federation of European Biochemical Societies.