Metabolic Profiling of Developing Pear Fruits Reveals Dynamic Variation in Primary and Secondary Metabolites, Including Plant Hormones.
Metabolic Profiling of Developing Pear Fruits Reveals Dynamic Variation in Primary and Secondary Metabolites, Including Plant Hormones.
复制标题
DOI：
10.1371/journal.pone.0131408
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Shiratake K
中科院分区：
文献类型：
--
作者：
Oikawa A;Otsuka T;Nakabayashi R;Jikumaru Y;Isuzugawa K;Murayama H;Saito K;Shiratake K
可食用植物果实中的代谢物包括甜味糖、视觉上吸引人的色素、具有人类营养价值的各种产品和具有生物活性的植物激素。虽然这些代谢物的数量在果实发育和成熟过程中因细胞分裂和扩大而变化，但很少有报道描述这些变化的实际动态。因此，我们应用多种代谢组学技术来鉴定梨果实发育和成熟过程中代谢物水平的变化。《法兰西》）。我们定量和分类超过250种代谢物分为六组，这取决于它们在发育和成熟过程中的特定变化模式。大约一半的代谢物，包括组氨酸和苹果酸，积累短暂的开花期，在此期间，细胞正在积极分裂，然后迅速或缓慢地减少。此外，含硫氨基酸的数量也增加了梨果实开花后3-4个月左右，当果实细胞扩大，但几乎消失，从成熟的果实。一些代谢物，包括植物激素脱落酸，积累特别是在花托开花和/或果实成熟之前。我们的研究结果表明，在发育和成熟梨果实的代谢产物水平的变化的几种模式，并提供基本的代谢组学数据，是有用的，了解梨果实生理和提高新品种的营养性状。
Metabolites in the fruits of edible plants include sweet sugars, visually appealing pigments, various products with human nutritional value, and biologically active plant hormones. Although quantities of these metabolites vary during fruit development and ripening because of cell division and enlargement, there are few reports describing the actual dynamics of these changes. Therefore, we applied multiple metabolomic techniques to identify the changes in metabolite levels during the development and ripening of pear fruits (Pyrus communis L. ‘La France’). We quantified and classified over 250 metabolites into six groups depending on their specific patterns of variation during development and ripening. Approximately half the total number of metabolites, including histidine and malate, accumulated transiently around the blooming period, during which cells are actively dividing, and then decreased either rapidly or slowly. Furthermore, the amounts of sulfur-containing amino acids also increased in pear fruits around 3–4 months after the blooming period, when fruit cells are enlarging, but virtually disappeared from ripened fruits. Some metabolites, including the plant hormone abscisic acid, accumulated particularly in the receptacle prior to blooming and/or fruit ripening. Our results show several patterns of variation in metabolite levels in developing and ripening pear fruits, and provide fundamental metabolomic data that is useful for understanding pear fruit physiology and enhancing the nutritional traits of new cultivars.