DNA analysis based on physical manipulation
DNA analysis based on physical manipulation
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基于物理操作的 DNA 分析
DOI：
10.1109/memsys.2000.838535
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
M. Washizu
中科院分区：
文献类型：
--
作者：
O. Kurosawa;K. Okabe;M. Washizu
提出了一种基于物理分子操作的DNA分析新方法。在玻璃基底上沉积牺牲层、载体层和一对电极，DNA溶液被供给到电极上。通过施加高频电压，DNA被拉伸成直线并对齐，其中一个分子末端锚定在电极边缘上，并固定在载体层上。然后使用AFM触针作为刀，将DNA的一部分与载体层一起切割，并通过溶解牺牲层，回收到膜过滤器上。使用PCR证明成功回收。
A novel DNA analysis method based on physical molecular manipulation is proposed and experimentally demonstrated. On a glass substrate is deposited a sacrificial layer, carrier layer and a pair of electrodes, onto which the DNA solution is fed. By applying high frequency voltage, the DNA is stretched straight and aligned, with one of its molecular end anchored on the electrode edge, and immobilized onto the carrier layer. Then using an AFM stylus as a knife, a portion of the DNA together with the carrier layer is cut, and by dissolving the sacrificial layer, recovered onto a membrane filter. Successful recovery is demonstrated using PCR.