Potential non-T cells source of interleukin-4 in food allergy.
Potential non-T cells source of interleukin-4 in food allergy.
复制标题
食物过敏中白细胞介素 4 的潜在非 T 细胞来源。
DOI：
10.1111/pai.12207
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Sampson,HughA
中科院分区：
文献类型：
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作者：
Caubet,Jean-Christoph;Masilamani,Madhan;Rivers,NeishaA;Mayer,Lloyd;Sampson,HughA
最近，食物过敏研究联盟(COFAR)的一项研究表明，过敏患者CD25+单个核细胞中变应原诱导的IL-4表达增加。然而，他们没有发现GATA-3表达的预期增加，这表明过敏原诱导的IL-4可能不是T细胞起源的。我们试图确定其他细胞类型是否与CD25+细胞群中IL-4表达增加有关。方法对比6名过敏患者和6名健康对照，我们分析了从PBMC分离的CD25+人群中是否存在潜在的IL-4表达的非T细胞。我们还比较了42名花生过敏患者和12名非特应性对照的嗜碱性粒细胞表面标志(CD203c、CD63、CD25和HLA-DR)的自发表达水平。结果在CD25+分离的细胞群中，除了CD4+T细胞外，还发现了相当大比例的非T细胞：嗜碱性粒细胞、NK细胞和NK-T细胞，平均百分比为±3.S.E.M.分别为5.24%±0.63%、6.65%±1.01%和6.01%±1.04%。其中大多数细胞胞浆内IL-4染色阳性。CD63和CD25在变态反应性疾病患者中的表达明显高于对照组(p<；0.05)。有趣的是，我们发现活化的嗜碱性细胞表达人类白细胞抗原-DR的比例明显高于未活化的嗜碱性细胞(p<；0.05)。结论我们的结果支持非T细胞分泌IL-4在食物过敏中的关键作用，特别是嗜碱性细胞，它可能在抗原呈递中发挥核心作用。
BackgroundRecently, a study from the Consortium of Food Allergy Research (CoFAR) showed that allergen‐inducedIL‐4expression in CD25+mononuclear cells was increased in allergic patients. However, they did not find the expected increase inGATA‐3expression, suggesting that allergen‐induced IL‐4 might not be of T‐cell origin. We sought to determine whether other cell types were responsible for the increasedIL‐4expression in the CD25+cell population.MethodsComparing six allergic patients and six healthy controls, we analyzed the CD25+isolated population from PBMC for the presence of potential IL‐4‐expressing non‐T cells. We also compared spontaneous expression levels of surface markers (CD203c, CD63, CD25, and HLA‐DR) on basophils from whole blood of 42 peanut‐allergic patients and from 12 non‐atopic controls. Expression of these markers was also evaluated following basophil activation in eight peanut‐allergic patients selected from the previous cohort.ResultsIn addition to CD4+T cells, a substantial proportion of non‐T cells were found in the CD25+‐isolated cell population: basophils, NK, and NK‐T cells with a mean percentage ± s.e.m. of 5.24 ± 0.63%, 6.65 ± 1.01%, and 6.01 ± 1.04%, respectively. The majority of these cells exhibited positive intracytoplasmic staining for IL‐4. Expression of CD63 and CD25 was significantly higher in allergic patients compared with controls (p<0.05). Interestingly, we found a significantly higher proportion of activated basophils expressing HLA‐DR, compared with non‐activated basophils (p<0.05).ConclusionsOur results support the suggested key role of non‐T cells secreting IL‐4 in food allergy, particularly basophils, which may also play a central role in antigen presentation.