Demonstration of a frozen sample aliquotter to prepare plasma and serum aliquots without thawing frozen parent samples.
Demonstration of a frozen sample aliquotter to prepare plasma and serum aliquots without thawing frozen parent samples.
复制标题
演示冷冻样品等分器无需解冻冷冻母体样品即可制备血浆和血清等分样品。
DOI：
10.1089/bio.2012.0049
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发表时间：
2013
影响因子：
1.6
通讯作者：
Venturini,DeborahS
中科院分区：
文献类型：
--
作者：
Ellis,HelenaJudge;Venturini,DeborahS
人类生物标本是推进分子医学、流行病学和生物标志物发现/验证以及其他生物医学研究的宝贵资源。生物库通常冷冻保存生物标本，以保护其生化成分。然而，将标本反复暴露于冻融循环可能会以不可预见的方式降低其完整性。因此，储存液体样品的那些生物库在收集时经常在许多小体积的冷冻样品（例如，0.5 mL或更小）或以更少、更大的体积（例如，1.8 mL）。前者消除了将样品暴露于重复的冷冻/解冻循环的需要，尽管增加了前期劳动力成本、消耗品使用和冷藏空间要求。后者降低了前期劳动力成本、消耗品和冷藏要求，但当需要更小的等分试样进行分析时，会使样品反复暴露于破坏性的冻融循环。布朗大学的罗得岛生物银行（RIBB）彻底评估了一种原始技术的性能，该技术通过从单个冷冻母体样品中自动提取冷冻等分试样而无需解冻，从而最大限度地减少了样品暴露于冷冻/解冻循环的风险。一种消除不必要的样品暴露于冷冻/解冻循环的技术可以帮助保护样品完整性，延长其使用寿命，并有效纠正和消除上述妥协的需要。本报告提供了评价结果，并最终证明了该技术能够从单个冷冻管中提取多个均匀的冷冻等分试样的从未解冻的冷冻人血浆，当分析典型的生化分析物时，这些血浆忠实地代表母体样品，显示变异系数低于5.5%。
Human biospecimens represent invaluable resources to advance molecular medicine, epidemiology, and biomarker discovery/validation, among other biomedical research. Biobanks typically cryopreserve biospecimens to safeguard their biochemical composition. However, exposing specimens repeatedly to freeze/thaw cycles can degrade their integrity in unforeseen ways. Those biobanks storing liquid samples, thus, regularly make a fundamental compromise at collection time between freezing samples in many small volumes (e.g., 0.5 mL or smaller) or in fewer, larger volumes (e.g., 1.8 mL). The former eliminates the need to expose samples to repeated freeze/thaw cycling, although increasing up-front labor costs, consumables used, and cold storage space requirements. The latter decreases up-front labor costs, consumables, and cold storage requirements, yet exposes samples repeatedly to damaging freeze/thaw cycles when smaller aliquots are needed for analysis. The Rhode Island BioBank at Brown University (RIBB) thoroughly evaluated the performance of an original technology that minimizes a sample's exposure to freeze/thaw cycling by enabling the automated extraction of frozen aliquots from one single frozen parent sample without thawing it. A technology that eliminates unnecessary sample exposures to freeze/thaw cycles could help protect sample integrity, extend its useful life, and effectively rectify and eliminate the aforementioned need to compromise. This report presents the results of the evaluation, and conclusively demonstrates the technology's ability to extract multiple uniform frozen aliquots from a single cryotube of never-thawed frozen human plasma, which faithfully represent the parent sample when analyzed for typical biochemical analytes, showing a coefficient of variability lower than 5.5%.