Inactivation of the shutoff gene (UL41) of herpes simplex virus types 1 and 2.
Inactivation of the shutoff gene (UL41) of herpes simplex virus types 1 and 2.
复制标题
1 型和 2 型单纯疱疹病毒关闭基因 (UL41) 失活。
DOI：
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发表时间：
1990
影响因子：
3.8
通讯作者：
R. Everett
中科院分区：
文献类型：
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作者：
M. Fenwick;R. Everett
单纯疱疹病毒1型（HSV-1）的基因UL 41和HSV-2的相应基因控制病毒体介导的细胞蛋白质合成的早期抑制，它们通过将β-半乳糖苷酶表达盒插入它们的编码区而失活。所得到的重组体在组织培养中生长良好，尽管2型重组病毒蛋白质合成稍微延迟。由于UL 41宿主蛋白质合成的失活，在放线菌素的存在下或在正常感染的早期没有受到抑制，尽管它在后期有所下降。多核糖体在感染早期没有被分解，细胞DNA合成没有被抑制，并且在放线菌酮存在下，稳定的α（立即早期）mRNA积累，与亲本HSV-2菌株形成鲜明对比。HSV-1和关闭缺陷型重组病毒的纯化病毒体的蛋白质的比较揭示了与包膜病毒体中存在UL 41基因产物一致的差异。
Gene UL41 of herpes simplex virus type 1 (HSV-1) and the corresponding gene of HSV-2, which control the virion-mediated early suppression of cellular protein synthesis, have been inactivated by inserting a beta-galactosidase expression cassette into their coding regions. The resulting recombinants grew well in tissue culture, although with the type 2 recombinant viral protein synthesis was slightly delayed. As a result of inactivation of UL41 host protein synthesis was not suppressed in the presence of actinomycin or early in normal infection, although it declined at a late stage. Polyribosomes were not broken down early in infection, cellular DNA synthesis was not inhibited and in the presence of cycloheximide stable alpha (immediate early) mRNA accumulated, in marked contrast to that of the parent HSV-2 strain. Comparison of the proteins of purified virions of HSV-1 and shutoff-defective recombinant virus revealed discrepancies consistent with the presence of the UL41 gene product in the enveloped virion.