Increased Energy Expenditure in Gastric Bypass Rats Is Not Caused by Activated Brown Adipose Tissue
Increased Energy Expenditure in Gastric Bypass Rats Is Not Caused by Activated Brown Adipose Tissue
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DOI：
10.1159/000339742
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发表时间：
2012-06-01
期刊：
影响因子：
3.6
通讯作者：
le Roux, Carel W.
中科院分区：
文献类型：
--
作者：
Hankir, Mohammed;Bueter, Marco;le Roux, Carel W.
目的：研究胃转流术是否诱导大鼠棕色脂肪组织活性升高和棕色脂肪组织特异性蛋白解偶联蛋白-1（UCP-1）水平升高。研究方法：胃旁路大鼠和假手术对照组（每组n = 8）进行全身H-1-MR波谱分析身体组成和F-18-氟脱氧葡萄糖正电子发射断层扫描结合计算机断层扫描（F-18-FDG PET/CT）成像测量棕色脂肪组织的代谢活性。收集棕色脂肪组织并称重，通过北方印迹技术测定UCP-1 mRNA的含量。结果：与假手术大鼠相比，胃旁路大鼠全身脂肪组织质量的百分比显著降低（p = 0.001）。两组之间的棕色脂肪组织活性无差异（假手术组的标准化摄取值为2.81 +/- 0.58，旁路组为2.56 +/- 0.46; p = 0.73）。此外，两组之间棕色脂肪组织的UCP-1 mRNA含量没有差异（假手术组49.5 +/- 13.2 vs.旁路组43.7 +/- 13.1; p = 0.77）。结论：胃旁路术并不增加大鼠棕色脂肪组织的活性，这表明旁路手术后能量消耗增加的原因还涉及其他机制。我们的结果不能证明F-18-FDG PET/CT研究的辐射剂量是合理的，以确定胃旁路手术后棕色脂肪组织的潜在变化。版权所有（C）2012 S. Karger GmbH，弗赖堡
Objective: To investigate whether gastric bypass induces a higher activity of brown adipose tissue and greater levels of the brown adipose tissue-specific protein uncoupling protein-1 (UCP-1) in rats. Methods: Gastric bypass rats and sham-operated controls (each n = 8) underwent whole body H-1-MR spectroscopy for analysis of body composition and F-18-fluorodeoxyglucose positron emission tomography combined with computed tomography (F-18-FDG PET/CT) imaging for measurement of the metabolic activity of brown adipose tissue. Brown adipose tissue was harvested and weighed, and UCP-1 mRNA content was measured by Northern Blot technique. Results: Gastric bypass rats had a significantly lower percentage of whole body adipose tissue mass compared to sham-operated rats (p = 0.001). There was no difference in brown adipose tissue activity between the two groups (standardised uptake value sham 2.81 +/- 0.58 vs. bypass 2.56 +/- 0.46; p = 0.73). Furthermore, there was no difference in the UCP-1 mRNA content of brown adipose tissue between the two groups (sham 49.5 +/- 13.2 vs. bypass 43.7 +/- 13.1; p = 0.77). Conclusion: Gastric bypass does not increase the activity of brown adipose tissue in rats suggesting that other mechanisms are involved to explain the increased energy expenditure after bypass surgery. Our results cannot justify the radiation dose of F-18-FDG PET/CT studies in humans to determine potential changes in brown adipose tissue after gastric bypass surgery. Copyright (C) 2012 S. Karger GmbH, Freiburg