EXTENSIVE REGULATORY CAPABILITIES OF A DROSOPHILA IMAGINAL DISK BLASTEMA
EXTENSIVE REGULATORY CAPABILITIES OF A DROSOPHILA IMAGINAL DISK BLASTEMA
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DOI：
10.1038/294744a0
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发表时间：
1981-01-01
期刊：
影响因子：
64.8
通讯作者：
SCHUBIGER, G
中科院分区：
文献类型：
--
作者：
KARPEN, GH;SCHUBIGER, G
每个有机体都会发展出在同一物种的个体之间准确重复的身体模式。为了了解细胞如何相互作用形成这些模式，许多研究人员研究了遗传和/或物理扰动后模式的重建。在调节系统中，缺失的结构可能被细胞增殖（外态）或不生长的重特异性（形态轴）所取代1。外态涉及胚基的形成和生长，对于脊椎动物再生来说，胚基的定义是位于伤口表面附近的增殖细胞区域，即使与肢体的其他部分隔离，也能够再生。果蝇成虫盘碎片的克隆分析表明，在模式调节过程中，大多数分裂细胞位于伤口表面附近5。此外，有丝分裂像（L.C. Abbott，个人通讯）和掺入 3 H-胸苷 6 的细胞聚集在调节片段的伤口表面附近。在这里，我们描述了涉及该增殖细胞区域培养物的实验，并报告它可以单独调节。这些观察提供的证据表明，盘碎片的调节是通过胚基的生长来完成的。令人惊讶的是，孤立的芽基再生的模式元素比极坐标模型预测的要多。我们通过提出在自由切割边缘启动调节来解决这个悖论，其中形成胚基，在序列中添加新的位置值。伤口愈合完成后，芽基继续生长，直到通过插入消除剩余的位置差异，如极坐标模型中所示。
Every organism develops body patterns that are accurately repeated among individuals of the same species. To understand how cells interact to form these patterns, many investigators have studied the re-establishment of patterns following genetic and/or physical perturbations. In regulating systems, missing structures may be replaced by cell proliferation (epimorphosis) or by respecification without growth (morphallaxis)1. Epimorphosis involves the formation and growth of a blastema2, defined for vertebrate regeneration as a zone of proliferating cells, localized near the wound surface3, which becomes capable of regenerating even when isolated from the rest of the limb4. Clonal analysis ofDrosophilaimaginal disk fragments indicates that during pattern regulation most dividing cells are located near the wound surface5. Furthermore, mitotic figures (L. C. Abbott, personal communication) and cells incorporating3H-thymidine6are clustered near the wound surface of regulating fragments. Here we describe experiments involving cultures of this zone of proliferating cells, and report that it can regulate in isolation. These observations provide evidence that regulation of disk fragments is accomplished by growth of a blastema. Surprisingly, the isolated blastema regenerated more pattern elements than predicted by the polar coordinate model7. We resolve this paradox by proposing that regulation is initiated at free cut edges, where a blastema is formed that adds new positional values in a sequence. On completion of wound healing, blastema growth continues until remaining positional disparities are eliminated by intercalation, as presented in the polar coordinate model.