Demonstration of the absence of intervening sequences within 23S rRNA genes from Campylobacter lari
Demonstration of the absence of intervening sequences within 23S rRNA genes from Campylobacter lari
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DOI：
10.1002/jobm.200800232
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发表时间：
2009-08-01
影响因子：
3.1
通讯作者：
Matsuda, Motoo
中科院分区：
文献类型：
--
作者：
Tazumi, Akihiro;Kakinuma, Yuki;Matsuda, Motoo
使用两个新型 PCR 引物对对 12 种脲酶阳性嗜热弯曲杆菌 (UPTC) 分离株中的近全长 23S rRNA 基因进行了克隆、测序和表征。首先显示来自 12 个分离株的 23S rRNA 基因的核苷酸序列在 25 和 45 螺旋区域均不携带任何插入序列 (IVS)。然后，在计算机上设计了两个 PCR 引物组，用于扩增来自 Lari 弯曲杆菌的 23S rRNA 基因序列内的螺旋 25 和 45 区域。经过 PCR 扩增、TA 克隆和测序程序后，在总共 53 个 C. lari 分离株的 23S rRNA 基因中未发现 IVS。在所有 63 个 C. lari 分离株中均鉴定出完整的 23S rRNA，导致不产生片段化的 23S rRNA。这些数据表明，C. lari 到目前为止可能没有任何机会与任何其他来源的 IVS 相互作用，或者无法将 IVS 整合到自己的基因组中。
Cloning, sequencing and characterization of nearly full-length 23S rRNA genes in 12 urease-positive thermophilic Campylobacter (UPTC) isolates were carried out using two novel PCR primer pairs. Nucleotide sequences of the 23S rRNA genes from the 12 isolates were first shown not to carry any intervening sequences (IVSs) in both the 25 and 45 helix regions. Then, two PCR primer sets were designed in silico for amplification of the helix 25 and 45 regions within 23S rRNA gene sequences from Campylobacter lari. No IVSs were identified within the 23S rRNA genes among a total of 53 isolates of C. lari, following PCR amplification, TA cloning and sequencing procedures. Intact 23S rRNA was identified in all 63 C. lari isolates, resulting in no production of the fragmented 23S rRNA. These data suggest that C. lari may not have any opportunity to interact with any other source of IVSs until now, or has been unable to integrate IVSs into their own genomes.