Dehydration‐induced proliferation of identified pituicytes in fully adult rats
Dehydration‐induced proliferation of identified pituicytes in fully adult rats
复制标题
成年大鼠脱水诱导的垂体细胞增殖
作者：
P. Murugaiyan;A. Salm
有丝分裂活性星形胶质细胞在成人中枢神经系统（CNS）的利益，由于其在神经胶质增生和肿瘤发生的作用，和他们的潜力，在帮助恢复功能损伤或疾病。垂体后叶提供了一个潜在的强大的模型来研究体内增殖，因为其居民星形胶质细胞，称为垂体细胞，已被报道分裂同时从神经分泌终端的激素释放。我们在这项研究中的目的是确认和表征这种增殖反应在脱水和补液完全成年动物，使用当代技术。成年雄性大鼠给予2%盐水代替水0-9天。在用增殖标记物5-溴脱氧尿苷（BrdU）、星形胶质细胞标记物胶质细胞酸性蛋白（GFAP）和DNA标记物4，6，二脒基-2-苯基吲哚，HCl（DAPI）三重标记的组织切片中定量垂体细胞的增殖。一个强大的增殖反应开始在三天内脱水，并继续以恒定的速度之后。在允许再水化的动物中，这种反应持续存在。脱水9天后，约35%的垂体细胞参与有丝分裂。虽然细胞密度在各种条件下保持恒定，但观察到垂体后叶面积可逆性增加，表明一些细胞死亡也同时发生。很大一部分非垂体细胞也发生了类似的变化。这些结果表明，垂体细胞在成人垂体后叶保留必要的特性，重新进入细胞周期，在神经分泌活动的局部因素。我们推测，这种增殖反应是直接相关的形态学变化，这些细胞在活化条件下，以前的报告。© 1995 Wiley利斯公司
The mitotic activity astrocytes in the adult central nervous system (CNS) is of interest due to their roles in gliosis and tumorigenesis, and their potential in aiding recovery of function following injury or disease. The posterior pituitary offers a potentially powerful model to study proliferation in vivo, since its resident astrocytes, called pituicytes, have been reported to divide concurrently with hormone release from the neurosecretory terminals there. Our aim in this study was to confirm and characterize this proliferative response during dehydration and rehydration in fully adult animals, using contemporary techniques. Adult male rats were given 2% saline in substitution for water for 0–9 days. Proliferation of pituicytes was quantified in tissue sections triple‐labeled with the proliferation marker, 5‐bromodeoxyuridine (BrdU), the astrocyte marker glial fibrillary acidic protein (GFAP), and the DNA marker 4,6, diamidino‐2‐phenylindole, HC1 (DAPI). A robust proliferative response began within three days of dehydration and continued at a constant rate thereafter. In animals allowed to rehydrate, this response continued. After 9 days of dehydration, approximately 35% of pituicytes had participated in mitosis. While cell density remained constant across conditions, a reversible increase in posterior pituitary area was seen, suggesting that some cell death also occurs simultaneously. A significant proportion of non‐pituicytes also underwent similar changes. These results indicate that pituicytes in the adult posterior pituitary retain characteristics necessary for reentering the cell cycle in response to local factors present during neurosecretory activity. We hypothesize that this proliferative response is directly related to the morphological changes previously reported for these cells under activating conditions. © 1995 Wiley‐Liss, Inc.
再生抑制：背根过渡区反应性星形胶质细胞和失效轴突末端的超微结构。
影响因子：
--
作者：
Stensaas,LJ;Partlow,LM;Burgess,PR;Horch,KW
通讯作者：
Horch,KW
加压素刺激培养物中肾上皮细胞的生长。
DOI：
10.1152/ajpcell.1983.245.5.c365
发表时间：
1983
期刊：
The American journal of physiology
影响因子：
--
作者：
Walsh-Reitz,MM;Toback,FG
通讯作者：
Toback,FG