A GROWTH-RELATED MESSENGER-RNA IN CULTURED MOUSE CELLS ENCODES A PLACENTAL CALCIUM-BINDING PROTEIN
A GROWTH-RELATED MESSENGER-RNA IN CULTURED MOUSE CELLS ENCODES A PLACENTAL CALCIUM-BINDING PROTEIN
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DOI：
10.1093/nar/15.16.6677
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发表时间：
1987-08-25
影响因子：
14.9
通讯作者：
LINZER, DIH
中科院分区：
文献类型：
--
作者：
JACKSONGRUSBY, LL;SWIERGIEL, J;LINZER, DIH
我们的特点是mRNA增加后，大量血清刺激静止小鼠成纤维细胞。该mRNA命名为18A2，编码与已知钙结合蛋白同源的101个氨基酸的预测多肽。多种小鼠组织表达18A2 mRNA，在非妊娠子宫和胎盘中检测到最高水平。总胎盘RNA中18A2 mRNA的浓度从妊娠第8天至第10天降低，并且在整个妊娠后半期低于检测。在血清刺激的成纤维细胞中，18A2 mRNA的增加依赖于蛋白质合成。18A2 mRNA在大小、血清诱导和序列上与2A9 mRNA相似（1），但这些mRNA来自不同的基因。这表明小鼠基因组中含有一个血清诱导型基因家族，该家族编码的蛋白质预计与钙结合。
We have characterized an mRNA that increases in abundance after serum stimulation of quiescent mouse fibroblasts. This mRNA, designated 18A2, encodes a predicted polypeptide of 101 amino acids with homology to known calcium binding proteins. A variety of mouse tissues express the 18A2 mRNA, with the highest levels detected in the non-pregnant uterus and in the placenta. The concentration of 18A2 mRNA in total placental RNA decreases from day 8 to day 10 of pregnancy, and is below detection throughout the latter half of gestation. In serum-stimulated fibroblasts, the increase in 18A2 mRNA is dependent on protein synthesis. The 18A2 mRNA is similar in size, serum-inducibility, and sequence to the 2A9 mRNA (1), but these mRNAs are derived from distinct genes. This suggests that the mouse genome harbors a family of serum-inducible genes encoding proteins predicted to bind calcium.