Caloric restriction and physical activity in zebrafish (Danio rerio)
Caloric restriction and physical activity in zebrafish (Danio rerio)
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DOI：
10.1016/j.neulet.2005.03.048
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发表时间：
2005-07-22
影响因子：
2.5
通讯作者：
Levine, JA
中科院分区：
文献类型：
--
作者：
Novak, CM;Jiang, XL;Levine, JA
了解能量流的机制对于解释肥胖如何成为一种公共卫生流行病至关重要。已知热量摄入的变化可预测地改变哺乳动物的体力活动水平（PA）。在这里，我们的目标是通过测量斑马鱼长期食物剥夺前后的PA来检验禁食诱导PA变化的双相模式的假设。与对照组相比，食物剥夺组的鱼在食物剥夺的前2天PA水平显着增加。然而，随后，禁食的鱼表现出显着的慢性减少PA相比，鱼喂养的体重维持水平。这些数据与哺乳动物的数据相当，哺乳动物也显示PA对热量限制的双相反应。在一个单独的组的鱼，长期的食物剥夺，与PA的减少，诱导大脑preproorexin mRNA水平显着增加相比，美联储控制。禁食2天后，食欲素mRNA没有变化。食欲素mRNA表达仅在长期饥饿后改变的发现表明，食欲素可能与此时观察到的PA变化相关联。因此，负能量平衡和PA减少之间的关联在生物学中跨属发生，并且与脑基因表达的可预测神经学变化相关。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
Understanding the mechanism of energy flux may be critical for explaining how obesity has emerged as a public health epidemic. It is known that changes in caloric intake predictably alter physical activity levels (PA) in mammals. Here, our goal was to test the hypothesis that fasting induces a biphasic pattern of change in PA by measuring PA before and after long-term food deprivation in zebrafish. Compared to control-fed fish, food-deprived fish showed a significant increase in PA levels during the first 2 days of food deprivation. Subsequently, however, fasted fish showed a significant chronic decrease in PA compared to fish fed at weight-maintenance levels. These data are comparable to those seen with mammals, which also show a biphasic response of PA to caloric restriction. In a separate group of fish, long-term food deprivation, associated with decreases in PA, induced a significant increase in brain preproorexin mRNA levels compared to fed controls. No change in orexin mRNA was seen after 2 days of food deprivation. The finding that orexin mRNA expression is altered only after long-term starvation suggests that orexin may be coupled with the changes in PA seen at this time. Thus, the association between negative energy balance and reductions in PA occurs across genera in biology and is associated with predictable neurological changes in brain gene expression. (c) 2005 Elsevier Ireland Ltd. All rights reserved.