Protein-synthesis-dependent induction of annexin I by glucocorticoid.
Protein-synthesis-dependent induction of annexin I by glucocorticoid.
复制标题
糖皮质激素对膜联蛋白 I 的蛋白质合成依赖性诱导。
DOI：
10.1042/bj2750313
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Nick,HS
中科院分区：
文献类型：
--
作者：
Wong,WT;Frost,SC;Nick,HS
We demonstrate that annexin I/lipocortin I (lipo I) gene expression is regulated by dexamethasone (DEX) in mouse 3T3-L1 fibroblasts and LA-4 lung epithelial cells. We have characterized this induction further in 3T3-L1 fibroblasts. At 24 h after addition of DEX, the levels of lipo I mRNA and protein increased 5-fold and 1.5-fold respectively. Time-course experiments revealed that the induction was delayed by 2-4 h after DEX addition. Half-maximal induction of both lipo I mRNA and protein was achieved with 10 nM-DEX. Both actinomycin D and cycloheximide blocked the DEX effect on lipo I mRNA expression. These results indicate that the induction of lipo I by DEX has a transcriptional component and requires protein synthesis de novo.
