Porphyrins as spectroscopic sensors for conformational studies of DNA
Porphyrins as spectroscopic sensors for conformational studies of DNA
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DOI：
10.1351/pac200779040801
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发表时间：
2007-04-01
影响因子：
1.8
通讯作者：
Berova, Nina
中科院分区：
文献类型：
--
作者：
Balaz, Milan;Bitsch-Jensen, Klaus;Berova, Nina
含有两个或多个相互作用的卟啉的分子系统显示出显着的光谱特征，允许通过不同的方法，如荧光和电子圆二色性（ECD）在微观水平上的构象变化的非常灵敏的检测。共价卟啉-DNA组装体可以在卟啉Soret带区域内提供CD分布（激子偶联），这对于DNA构象变化非常有诊断意义。此外，共价连接的卟啉已经显示出作为DNA分子帽的功能，并通过其强π-π堆积来稳定非自身互补的非沃森-克里克鸟嘌呤-腺嘌呤DNA序列。
Molecular systems containing two or more interacting porphyrins show remarkable spectroscopic features that allow for a very sensitive detection of conformational changes on the microscale level by different methods, such as fluorescence and electronic circular dichroism (ECD). Covalent porphyrin-DNA assemblies can provide a CD profile (exciton couplet) within the porphyrin Soret band region which is very diagnostic for DNA conformational changes. Additionally, covalently linked porphyrins have been shown to function as DNA molecular caps and to stabilize the non-self-complementary non-Watson-Crick guanine-adenine DNA sequence via their strong pi-pi stacking.