High throughput sequencing analysis of the joint effects of BDE209-Pb on soil bacterial community structure.
High throughput sequencing analysis of the joint effects of BDE209-Pb on soil bacterial community structure.
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DOI：
10.1016/j.jhazmat.2015.08.037
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发表时间：
2016-01
影响因子：
13.6
通讯作者：
Wei Zhang-;Lei Chen;Rong Zhang;Kuangfei Lin
中科院分区：
文献类型：
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作者：
Wei Zhang-;Lei Chen;Rong Zhang;Kuangfei Lin
十溴二苯醚(BDE209)和铅(Pb2+)是电子垃圾回收场的主要污染物。然而，联合暴露于这两种化学物质对土壤微生物的影响几乎还不清楚。为此，通过室内培养试验，研究了在60d的培养期间，土壤微生物生物量和活性以及细菌群落结构的变化。结果表明，单独暴露铅或苯并二恶英暴露后，土壤微生物量碳显著降低(p<；P<0.01)，土壤基础呼吸(SBR)和代谢商(QCO2)增加，而苯并二恶英对土壤微生物量碳影响不显著(p>；p>；P>0.05)。在Illumina MiSeq平台上进行的16S rRNA基因测序表明，共有49,405个有效序列广泛代表了微生物群落的多样性。在门和属的分类水平上进行的序列分析表明，所有土壤样本中共鉴定出11个门297个属，污染物的输入影响了细菌群落结构，表明变形杆菌、放线菌和酸杆菌是优势类群，大型细菌属和芽孢杆菌属在污染土壤中较为丰富。在所有样本中单独接触BDE209表明，与对照相比，BDE209的群落结构更相似。这些观察结果有助于更好地了解BDE209和铅联合暴露对EWRSs土壤中土著微生物的生态毒理效应。
Decabromodiphenyl ether (BDE209) and Lead (Pb) are the main pollutants at e-waste recycling sites (EWRSs). However, the impact on soil microorganism of joint exposure to the two chemicals remains almost unknown. Therefore, the indoor incubation tests were performed to determine the response of soil microbial biomass and activity as well as bacterial community structure in the presence of the two chemicals during 60 d incubation period. The results indicated that after Pb alone or BDE209-Pb exposure, soil microbial biomass C (Cmic) was significantly lower (p< 0.01), and soil basal respiration (SBR) and metabolic quotient (qCO2) were enhanced, while BDE209 barely resulted in significant influence (p> 0.05). 16S rRNA gene sequencing on the Illumina MiSeq platform demonstrated that a total 49,405 valid sequences widely represented the diversity of microbial community. Sequence analyses at phylum and genus taxonomic levels illustrated that 11 identified phyla and 297 genera were observed among all the soil samples, and the contaminants input had affected bacterial community structure, suggesting that Proteobacteria, Actinobacteria and Acidobacteria were the dominant phyla, and the generaMassiliaandBacilluswere enriched in contaminated soil. BDE209 exposure alone in all the samples indicated a more similar community structure compared to the control. The results of these observations have provided a better understanding of ecotoxicological effects of BDE209 and Pb joint exposure on indigenous microorganisms in soil at EWRSs.