Role of PCR for diagnosing Pneumocystis jirovecii pneumonia in HIV-infected individuals in a tertiary care hospital in India
Role of PCR for diagnosing Pneumocystis jirovecii pneumonia in HIV-infected individuals in a tertiary care hospital in India
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DOI：
10.4103/0377-4929.81624
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发表时间：
2011-04-01
影响因子：
1
通讯作者：
Bairy, Indira
中科院分区：
文献类型：
--
作者：
Chawla, Kiran;Martena, Suganthi;Bairy, Indira
目的：在印度等发展中国家，通常通过常规染色或临床诊断来诊断乙氏肺囊虫感染。本研究旨在了解聚合酶链反应（FOR）在诊断人类免疫缺陷病毒（HIV）感染患者的乙基肺囊虫肺炎（PJP）中的应用，比较聚合酶链反应（FOR）结果与染色技术的比较，并将结果与患者的临床状况联系起来。材料与方法：一项前瞻性研究纳入了50例有下呼吸道感染症状的hiv感染成人住院患者。采用诱导痰、支气管肺泡灌洗或气管抽吸法对耶氏螺旋体线粒体大亚基rRNA基因进行染色和for检测。结果：在我们的研究中，14%（7/50）的病例中，FOR结果与染色结果相关。另有20%（10/50）的病例仅能诊断为FOR，其染色为阴性。与疾病的临床证据相比，FOR的敏感性为93.7%，特异性为94.1%。呼吸困难、CD4计数差异有统计学意义(P
Objectives: In developing countries like India, the diagnosis of Pneumocystis jirovecii infection is often made either by conventional staining or clinically. This study was planned to know the utility of polymerase chain reaction (FOR) in diagnosing Pneumocystis jirovecii pneumonia (PJP) in human immunodeficiency virus (HIV)-infected patients, to compare the FOR results with that of staining techniques and also to correlate the results with clinical condition of patients. Materials and Methods: A prospective study included 50 HIV-infected adult in-patients with symptoms of lower respiratory tract infection. Induced sputum, bronchoalveolar lavage or tracheal aspirate were proceeded for both staining and FOR for mitochondrial large subunit rRNA gene of P. jirovecii. Results: In our study FOR results correlated with staining findings in 14% (7/50) of cases. Another 20% (10/50) cases could be diagnosed only with FOR, where staining was negative for the presence of P. jirovecii. When compared with clinical evidence of disease, FOR showed 93.7% sensitivity and 94.1% specificity. Presence of dyspnea and CD4 count showed statistical significance (P