Preferential expression of a HLP homolog encoding a mitochondrial L14 ribosomal protein in stamens of common wheat
Preferential expression of a HLP homolog encoding a mitochondrial L14 ribosomal protein in stamens of common wheat
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DOI：
10.1016/j.gene.2004.09.005
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发表时间：
2004-12-22
期刊：
影响因子：
3.5
通讯作者：
Takumi, S
中科院分区：
文献类型：
--
作者：
Mizumoto, K;Murai, K;Takumi, S
细胞核与细胞质的相互作用在植物的发育过程中起着重要的作用，包括花器官的发育。我们分离了拟南芥HUELLENLOS PARALOG（HLP）基因编码的线粒体（mt）核糖体蛋白L14的小麦同源物Whlp。利用绿色荧光蛋白（GFP）融合蛋白的瞬时表达分析表明，位于小麦HLP同源蛋白（WHLP）N端的50个氨基酸残基作为线粒体靶向信号（NITS）。比较了小麦异源细胞质系花器官中Whlp基因的表达模式，其中内在的细胞质被野生的相对粗厚山羊草的细胞质所取代。在这些异质系中，在没有核恢复基因的情况下，由外源细胞质诱导雌蕊化（雄蕊同源异型转化为雌蕊样器官）。与雌蕊、叶和根相比，鞭转录本优先在雄蕊中积累。Whip在异源质系雌蕊中的表达水平与真雌蕊和可育异源质系雌蕊中的表达水平相似。结果表明，Whlp基因的高表达对雄性生殖器官的发育起辅助作用，但不决定其轮性。在另一个核编码的mt核糖体蛋白基因中观察到类似的表达模式，但在mt编码的基因中没有。不同lilt核糖体蛋白基因的不同表达模式表明，小麦器官/组织发育中线粒体核糖体蛋白的丰度受到差异调节。(C)2004 Elsevier B. V.保留所有权利。
Interaction between nucleus and cytoplasm has essential roles in plant development, including that of floral organs. We isolated a wheat homolog Whlp of Arabidopsis HUELLENLOS PARALOG (HLP) gene encoding a mitochondrial (mt) ribosomal protein L14. Transient expression analysis using the green fluorescent protein (GFP) fusion protein showed that 50 amino residues located on the N-terminal of the wheat HLP homolog (WHLP) protein acted as a mt-targeting signal (NITS). Expression patterns of the Whlp gene were compared among floral organs of alloplasmic lines of wheat, in which intrinsic cytoplasms were replaced by the cytoplasm of a wild relative Aegilops crassa. In these alloplasmic lines, pistillody (homeotic transformation of stamens into pistil-like organs) is induced by the alien cytoplasm in the absence of nuclear restorer genes. The Whip transcripts preferentially accumulated in stamens compared with pistils, leaves, and roots. The expression level of Whip in the pistillate stamens of the alloplasmic lines was similar to that in genuine pistils of both euplasmic lines and fertile alloplasmic lines. The result suggested that the elevated expression of the Whlp gene plays a role in aiding the development of male reproductive organ but not in the determination of its whorl identity. A comparable expression pattern was observed in another nuclear-encoded mt ribosomal protein gene but not in a mt-encoded gene. The different expression patterns of different lilt ribosomal protein genes suggest that the abundance of mt ribosomal proteins is differentially regulated in the organ/tissue development in wheat. (C) 2004 Elsevier B.V. All rights reserved.