Extensive expansion of the claudin gene family in the teleost fish, Fugu rubripes
Extensive expansion of the claudin gene family in the teleost fish, Fugu rubripes
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DOI：
10.1101/gr.2400004
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发表时间：
2004-07-01
期刊：
影响因子：
7
通讯作者：
Venkatesh, B
中科院分区：
文献类型：
--
作者：
Loh, YH;Christoffels, A;Venkatesh, B
在人类中，claudin超家族由19种同源蛋白组成，通常定位于上皮细胞和内皮细胞的紧密连接。除了作为结构紧密连接组分外，claudin还参与细胞间粘附和溶质的细胞旁转运。在这里，我们确定和注释的全基因组中的硬骨鱼，红鳍东方（河豚）的claudin基因，并确定其系统发育关系的哺乳动物。我们的分析揭示了广泛的基因重复的硬骨鱼谱系，导致56 claudin基因在河豚。共有35个河豚claudin基因可以被分配到17个哺乳动物claudin基因的直系同源性，其余21个基因是特定的鱼类谱系。因此，大量的额外的河豚基因不是在鱼类谱系中提出的全基因组重复的结果。表达谱显示，56个河豚封闭蛋白基因中的大多数以或多或少的组织特异性方式或在特定的发育阶段表达。我们推测，在硬骨鱼类中claudin基因家族的扩展允许在进化过程中获得新的功能，并且鱼类特异性的基因家族的新成员，如claudins，在很大程度上有助于鱼类和哺乳动物的独特生理。
In humans, the claudin superfamily consists of 19 homologous proteins that commonly localize to tight junctions of epithelial and endothelial cells. Besides being structural tight-junction components, claudins participate in cell-cell adhesion and the paracellular transport of solutes. Here, we identify and annotate the claudin genes in the whole-genome of the teleost fish, Fugu rubripes (Fugu), and determine their phylogenetic relationships to those in mammals. Our analysis reveals extensive gene duplications in the teleost lineage, leading to 56 claudin genes in Fugu. A total of 35 Fugu claudin genes can be assigned orthology to 17 mammalian claudin genes, with the remaining 21 genes being specific to the fish lineage. Thus, a significant number of the additional Fugu genes are not the result of the proposed whole-genome duplication in the fish lineage. Expression profiling shows that most of the 56 Fugu claudin genes are expressed in a more-or-less tissue-specific fashion, or at particular developmental stages. We postulate that the expansion of the claudin gene family in teleosts allowed the acquisition of novel functions during evolution, and that fish-specific novel members of gene families Such as claudins contribute to a large extent to the distinct physiology of fishes and mammals.