Ultrasmall Superparamagnetic Iron Oxide Labeled Silk Fibroin/Hydroxyapatite Multifunctional Scaffold Loaded With Bone Marrow-Derived Mesenchymal Stem Cells for Bone Regeneration
Ultrasmall Superparamagnetic Iron Oxide Labeled Silk Fibroin/Hydroxyapatite Multifunctional Scaffold Loaded With Bone Marrow-Derived Mesenchymal Stem Cells for Bone Regeneration
复制标题
超小型超顺磁性氧化铁标记丝素蛋白/羟基磷灰石多功能支架装载骨髓源性间充质干细胞用于骨再生
DOI：
10.3389/fbioe.2020.00697
复制
发表时间：
2020-06-30
影响因子：
5.7
通讯作者：
Xu, Yikai
中科院分区：
文献类型：
--
作者：
Liu, Qin;Feng, Longbao;Xu, Yikai
许多组织工程构建体已经被研究作为再生医学中的骨支架。然而，无创监测骨移植后的生物降解和重塑仍然是一个挑战。本研究成功合成了丝素蛋白/羟基磷灰石复合纳米超顺磁性氧化铁（USPIO）支架，并对其进行了表征，并将其皮下植入裸鼠背部。USPIO标记支架具有良好的三维多孔结构和力学性能、热稳定性，可用于骨修复。加载骨髓间充质干细胞（BMSCs）后，多功能支架通过增加碱性磷酸酶活性和上调成骨基因，促进细胞粘附和生长，促进成骨。此外，体内定量磁共振成像（MRI）结果同时为支架降解和骨形成提供了有价值的信息，这一点得到了计算机断层扫描和组织学检查的进一步证实。这些结果表明，将USPIO结合到bmscs负载的多功能支架系统中，可以通过定量MRI无创监测骨再生。这种组织工程策略为骨缺损修复在临床中的转化应用提供了一种很有前途的工具。
Numerous tissue-engineered constructs have been investigated as bone scaffolds in regenerative medicine. However, it remains challenging to non-invasively monitor the biodegradation and remodeling of bone grafts after implantation. Herein, silk fibroin/hydroxyapatite scaffolds incorporated with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles were successfully synthesized, characterized, and implanted subcutaneously into the back of nude mice. The USPIO labeled scaffolds showed good three-dimensional porous structures and mechanical property, thermal stability for bone repair. After loaded with bone marrow-derived mesenchymal stem cells (BMSCs), the multifunctional scaffolds promoted cell adhesion and growth, and facilitated osteogenesis by showing increased levels of alkaline phosphatase activity and up-regulation of osteoblastic genes. Furthermore, in vivo quantitative magnetic resonance imaging (MRI) results provided valuable information on scaffolds degradation and bone formation simultaneously, which was further confirmed by computed tomography and histological examination. These findings demonstrated that the incorporation of USPIO into BMSCs-loaded multifunctional scaffold system could be feasible to noninvasively monitor bone regeneration by quantitative MRI. This tissue engineering strategy provides a promising tool for translational application of bone defect repair in clinical scenarios.