Albumin Nanoshell Encapsulation of Near-Infrared-Excitable Rare-Earth Nanoparticles Enhances Biocompatibility and Enables Targeted Cell Imaging
Albumin Nanoshell Encapsulation of Near-Infrared-Excitable Rare-Earth Nanoparticles Enhances Biocompatibility and Enables Targeted Cell Imaging
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DOI：
10.1002/smll.200902403
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发表时间：
2010-08-02
期刊：
影响因子：
13.3
通讯作者：
Moghe, Prabhas V.
中科院分区：
文献类型：
--
作者：
Naczynski, Dominik J.;Andelman, Tamar;Moghe, Prabhas V.
传统荧光团在体内成像应用中的使用受到量子产率差、激发光的组织穿透性差和过度的组织自身荧光的限制，而提供高量子产率的无机荧光颗粒的使用通常由于颗粒毒性而受到限制。利用近红外上转换的稀土掺杂纳米颗粒克服了传统荧光团的光学限制，但由于其在水溶液中的不溶性，通常不适合生物应用。缺乏用于偶联生物分子的功能表面基团，以及潜在的细胞毒性。报道了一种在920-980nm近红外光下可激发的稀土掺杂NaY F-4发光纳米壳配合物的制备方法。该方法包括将NaY F4纳米颗粒（与Yb和Er相连）封装在人血清白蛋白纳米壳中，以创建可水分散的、生物功能化的复合颗粒。这些颗粒的尺寸分布在200nm左右，在水溶液中稳定4周以上。白蛋白外壳具有细胞保护作用，即使浓度超过200 μ g REs mL也能显著提高REs的生物相容性（-1）。结合环精氨酸-甘氨酸-天冬氨酸（cRGD）的复合颗粒特异性靶向表达α (v) β(3)整合素受体的人胶质母细胞瘤细胞系和黑色素瘤细胞。这些发现强调了白蛋白包裹的稀土纳米颗粒在体外成像癌细胞和体内疾病部位靶向成像的潜力。
The use of traditional fluorophores for in vivo imaging applications is limited by poor quantum yield, poor tissue penetration of the excitation light, and excessive tissue autofluorescence, while the use of inorganic fluorescent particles that offer a high quantum yield is frequently limited due to particle toxicity. Rare-earth-doped nanoparticles that utilize near-infrared upconversion overcome the optical limitations of traditional fluorophores, but are not typically suitable for biological application due to their Insolubility in aqueous solution., lack of functional surface groups for conjugation of biomolecules, and potential cytotoxicity. A new approach to establish highly biocompatible and biologically targetable nanoshell complexes of luminescent rare-earth-doped NaY F-4 nanoparticles (REs) excitable with 920-980nm near-infrared light for biomedical imaging applications is reported. The approach involves the encapsulation of NaY F4 nanoparticles (loped with Yb and Er within human serum albumin nanoshells to create water-dispersible, biologically functionalizable composite particles. These particles exhibit narrow size distributions around 200nm and are stable in aqueous solution for over 4 weeks. The albumin shell confers cytoprotection and significantly enhances the biocompatibility of REs even at concentrations above 200 mu g REs mL(-1). Composite particles conjugated with cyclic arginine-glycine-aspartic acid (cRGD) specifically target both human glioblastoma cell lines and melanoma cells expressing alpha(v)beta(3) integrin receptors. These findings highlight the promise of albumin-encapsulated rare-earth nanoparticles for imaging cancer cells in vitro and the potential for targeted imaging of disease sites in vivo.