Behavior of 17D yellow fever virus in rhesus monkeys - Relation to substrain, dose and neural or extraneural inoculation
Behavior of 17D yellow fever virus in rhesus monkeys - Relation to substrain, dose and neural or extraneural inoculation
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DOI：
10.1093/oxfordjournals.aje.a118877
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发表时间：
1943-09-01
影响因子：
--
通讯作者：
Penna, HA
中科院分区：
文献类型：
--
作者：
Fox, JP;Penna, HA
对477只恒河猴（在单个批次疫苗的常规控制中进行脑内接种）的观察结果进行了17天接种病毒的亚株和剂量分析。8组猴接种17D病毒不同亚株的数据显示，在循环病毒的发生、免疫应答、发热反应的发作和持续时间以及脑炎表现的发生率方面存在显著差异，这些表现可表征各个亚株。这些差异是重要的，因为它们可以在一定程度上与亚株的能力，刺激免疫反应和脑炎反应在man.A研究也取得了病毒剂量之间的关系，循环病毒的发生，并在subcut的免疫反应。接种过疫苗的猴子在足够宽的病毒剂量范围内，显示出明确的关系。在最大剂量下，循环病毒几乎被抑制，而血清抗体可在第7天显示。用最小剂量接种后，直到第6或第7天才在循环中出现病毒，直到第11天后才能检测到血清抗体，尽管随后的滴度显著高于大剂量接种后的滴度。
Observations on 477 rhesus monkeys, inoculated intracerebrally in the routine control of individual lots of vaccine, were analyzed with respect to the substrain and dose of the 17d virus inoculated. The data for 8 groups of monkeys inoculated with different substrains of 17D virus reveal significant differences in the occurrence of circulating virus, in the immune response, in the onset and duration of febrile reactions, and in the incidence of encephalitic manifestations which serve to characterize the individual substrains. These differences are of importance since they can be correlated to some extent with the capacity of the substrains to stimulate both an immune response and encephalitic reactions in man. A study was made also of the relation between virus dose, the occurrence of circulating virus, and the immune response in subcut. inoculated monkeys. With a sufficiently wide range of virus doses, a clear-cut relation was shown. With the largest dose, circulating virus was almost suppressed while serum antibodies could be demonstrated by the 7th day. With the smallest dose, virus did not appear in the circulation until the 6th or 7th day and serum antibodies could not be detected until after the 11th day although the subsequent titcrs were significantly higher than those following the larger inocula.