Ribonuclease H: a Ubiquitous Activity in Virions of Ribonucleic Acid Tumor Viruses
Ribonuclease H: a Ubiquitous Activity in Virions of Ribonucleic Acid Tumor Viruses
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核糖核酸酶 H：核糖核酸肿瘤病毒病毒粒子中普遍存在的活性
作者：
D. Grandgenett;G. Gerard;M. Green
已检查了在五种不同宿主细胞种属中生长的十种核糖核酸（RNA）肿瘤病毒和来自三个不同病毒组的三种非致癌病毒的核糖核酸酶H含量。使用三种不同的底物测定核糖核酸酶H：小牛胸腺[3 H] RNA-脱氧核糖核酸（DNA）杂交物（由变性小牛胸腺DNA和大肠杆菌DNA指导的RNA聚合酶制备）、3 H-聚腺苷酸[3 H-聚（A）]与聚脱氧胸苷酸[poly（dT）]复合物以及3 H-聚尿苷酸[3 H-聚（U）]与聚脱氧腺苷酸[poly（dA）]复合物。所有10种RNA肿瘤病毒都含有核糖核酸酶H活性，其降解小牛胸腺杂交体和poly（A）-poly（dT）的RNA，而只有小鼠肉瘤白血病病毒的Moloney株和RD-猫白血病病毒中的核糖核酸酶H水解poly（U）-poly（dA）的RNA链。在流感病毒、仙台病毒或水疱性口炎病毒中未检测到明显的核糖核酸酶H活性。核糖核酸酶H和RNA指导的DNA聚合酶的活动在莫洛尼鼠肉瘤白血病病毒是不可分割的磷酸纤维素层析或甘油梯度离心，但似乎是部分分离的二乙基氨基乙基纤维素层析。
Ten ribonucleic acid (RNA) tumor viruses grown in five different host cell species and three non-oncogenic viruses from three different virus groups have been examined for ribonuclease H content. Three different substrates were used to assay ribonuclease H: calf thymus [3H]RNA-deoxyribonucleic acid (DNA) hybrid prepared with denatured calf thymus DNA and Escherichia coli DNA-directed RNA polymerase, 3H-polydenylic acid [3H-poly(A)] complexed to polydeoxythymidylic acid [poly(dT)], and 3H-polyuridylic acid [3H-poly(U)] complexed to polydeoxyadenylic acid [poly(dA)]. All ten RNA tumor viruses contained ribonuclease H activity which degraded the RNA of both the calf thymus hybrid and poly(A)-poly(dT), whereas only the ribonuclease H in the Moloney strain of murine sarcoma-leukemia virus and in RD-feline leukemia virus hydrolyzed the RNA strand of poly(U)-poly(dA). No appreciable ribonuclease H activity was detected in influenza, Sendai, or vesicular stomatitis virus. The ribonuclease H and RNA-directed DNA polymerase activities in Moloney murine sarcoma-leukemia virus were inseparable by phosphocellulose chromatography or glycerol gradient centrifugation, but appeared to be partially separated by diethylaminoethyl-cellulose chromatography.