Full crop protection from an insect pest by expression of long double-stranded RNAs in plastids
Full crop protection from an insect pest by expression of long double-stranded RNAs in plastids
复制标题
DOI：
10.1126/science.1261680
复制
发表时间：
2015-02-27
期刊：
影响因子：
56.9
通讯作者：
Bock, Ralph
中科院分区：
文献类型：
--
作者：
Zhang, Jiang;Khan, Sher Afzal;Bock, Ralph
针对必需基因的双链RNA(DsRNAs)可以在害虫中引发致命的RNA干扰(RNAi)反应。这一概念在植物保护中的应用受到内源植物RNAi途径的阻碍，该途径将dsRNAs处理成短干扰RNAs。我们发现，长的dsRNAs可以在叶绿体中稳定地产生，叶绿体是一个似乎缺乏RNAi机制的细胞室。当从叶绿体基因组表达dsRNAs时，dsRNAs累积到细胞总RNA的0.4%。转基因马铃薯植株产生针对科罗拉多州马铃薯甲虫-β-肌动蛋白基因的dsRNA，这是一种臭名昭著的农业害虫，可保护其免受草食性疾病的侵袭，并对其幼虫致死。因此，表达长dsRNA的叶绿体可以在没有化学农药的情况下提供作物保护。
Double-stranded RNAs (dsRNAs) targeted against essential genes can trigger a lethal RNA interference (RNAi) response in insect pests. The application of this concept in plant protection is hampered by the presence of an endogenous plant RNAi pathway that processes dsRNAs into short interfering RNAs. We found that long dsRNAs can be stably produced in chloroplasts, a cellular compartment that appears to lack an RNAi machinery. When expressed from the chloroplast genome, dsRNAs accumulated to as much as 0.4% of the total cellular RNA. Transplastomic potato plants producing dsRNAs targeted against the beta-actin gene of the Colorado potato beetle, a notorious agricultural pest, were protected from herbivory and were lethal to its larvae. Thus, chloroplast expression of long dsRNAs can provide crop protection without chemical pesticides.