Full-length sequence analysis of subacute sclerosing panencephalitis (SSPE) virus, a mutant of measles virus, isolated from brain tissues of a patient shortly after onset of SSPE
Full-length sequence analysis of subacute sclerosing panencephalitis (SSPE) virus, a mutant of measles virus, isolated from brain tissues of a patient shortly after onset of SSPE
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DOI：
10.1111/j.1348-0421.2006.tb03822.x
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发表时间：
2006-01-01
影响因子：
2.6
通讯作者：
Sada, Kiyonao
中科院分区：
文献类型：
--
作者：
Hotta, Hak;Nihei, Kenji;Sada, Kiyonao
亚急性硬化性泛脑炎(SSPE)病毒是一种麻疹病毒(MeV)变异株，从患者临床发病后不久的脑组织中分离出来，并对病毒全基因组进行了测序。该病毒命名为SSPE-Kobe-1，在B95a和Vero/SLAM细胞上形成合胞体，不产生无细胞感染性病毒颗粒，这是SSPE病毒的特征。系统发育分析表明，SSPE-Kobe-1属于D3型。SSPE-Kobe-1与同型MeV野毒株(Ich-B株)相比，在病毒基因组各蛋白编码区的核苷酸水平上显示出0.8-1.6%的突变率。值得注意的是，SSPE-Kobe-1株M基因的突变率(1.2%)明显低于已报道的其他SSPE病毒株，但大多数突变(75%)是SSPE病毒M基因的尿苷-胞苷偏向的过度突变特征。在氨基酸水平上，N、P、C、V、M、F、H和L蛋白分别在3、7、1、4、3、9、8和14个氨基酸残基发生点突变。此外，由于在终止密码子附近或在终止密码子上的单点突变，F和H蛋白发生了C末端突变。M蛋白的三个突变中有两个是Leu-to-Pro，这可能会影响蛋白质的构象，从而影响蛋白质的功能。由于SSPE-Kobe-1的突变数量相对较少，有望成为研究SSPE病毒遗传进化的有用工具。
Subacute sclerosing panencephalitis (SSPE) virus, a measles virus (MeV) mutant, was isolated from brain tissues of a patient shortly after the clinical onset, and the entire viral genome was sequenced. The virus, named SSPE-Kobe-1, formed syncytia on B95a and Vero/SLAM cells without producing cell-free infectious virus particles, which is characteristic of SSPE virus. Phylogenetic analysis classified SSPE-Kobe-1 into genotype D3. When compared with an MeV field isolate of the same genotype (Ich-B strain), SSPE-Kobe-1 exhibited mutation rates of 0.8-1.6% at the nucleotide level in each of the protein-coding regions of the viral genome. It is noteworthy that the mutation rate of the M gene (1.2%) of SSPE-Kobe-1 was considerably lower than for other SSPE virus strains reported so far, but that the majority of the mutations (75%) were the uridine-to-cytidine biased hypermutation characteristic of the SSPE virus M gene. At the amino acid level, the viral proteins, such as N, P, C, V, M, F, H and L proteins, had point-mutations on 3, 7, 1, 4, 3, 9, 8 and 14 residues, respectively, compared with the Ich-B strain. In addition, the F and H proteins had mutated C-termini due to single-point mutations near or at the stop codons. Two of the three mutations in the M protein were Leu-to-Pro, mutations, which are likely to affect the conformation and, therefore, the function of the protein. Because of the relatively small number of mutations, SSPE-Kobe-1 would be a useful tool to study genetic evolution of SSPE virus.