INACTIVATION OF ERYTHROPOIETIN BY NEURAMINIDASE AND BY MILD SUBSTITUTION REACTIONS
INACTIVATION OF ERYTHROPOIETIN BY NEURAMINIDASE AND BY MILD SUBSTITUTION REACTIONS
复制标题
DOI：
10.1038/185102a0
复制
发表时间：
1960-01-01
期刊：
影响因子：
64.8
通讯作者：
BORSOOK, H
中科院分区：
文献类型：
--
作者：
LOWY, PH;KEIGHLEY, G;BORSOOK, H
用苯肼制备的家兔血浆中红细胞生成活性与黏液蛋白1 - 4有关。研究表明，其活性可被蛋白水解酶1,3,5破坏。Rambachet等人和我们一样发现，在100°，pH值2下加热1小时。破坏活性，在豚鼠α1-酸性糖蛋白7的情况下，这种处理选择性地去除唾液酸。尽管这种处理很温和，但也有可能破坏了其他键（糖、氨基酸）。我们现在已经能够证明，接触神经氨酸酶8完全使黏液蛋白失活。
ERYTHROPOIETIC activity in the plasma of rabbits made anæmic with phenylhydrazine is associated with mucoproteins1–4. It has been shown that the activity is destroyed by proteolyfcic enzymes1,3,5. Rambachet al.6have found as well as us that heating at 100°,pH 2, for 1 hr. destroys the activity, a treatment which, in the case of guinea pig α1-acid glycoprotein7, selectively removed sialic acid. In spite of the mildness of this treatment it is possible that other bonds (sugar, amino-acid) were broken. We have now been able to show that exposure to neuraminidase8completely inactivates the mucoprotein.