In vivo tracing uptake and elimination of organic pesticides in fish muscle.
In vivo tracing uptake and elimination of organic pesticides in fish muscle.
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DOI：
10.1021/es5009032
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发表时间：
2014-06
影响因子：
11.4
通讯作者：
Jianqiao Xu;Junpeng Luo;Jingwen Ruan;Fang Zhu;Tiangang Luan;H. Liu;Ruifen Jiang;Gangfeng Ouyang-Gangfeng
中科院分区：
文献类型：
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作者：
Jianqiao Xu;Junpeng Luo;Jingwen Ruan;Fang Zhu;Tiangang Luan;H. Liu;Ruifen Jiang;Gangfeng Ouyang-Gangfeng
实验室测定的生物富集因子（BCFs）对于表征进入环境的化学品的生物累积特性具有重要意义，特别是潜在的持久性有机污染物（POPs），它会对生态系统和人类健康造成严重的不利影响。传统的致死分析方法耗时长，牺牲的实验动物过多。本研究采用固相微萃取（SPME）技术，在活体条件下对农药在鱼类体内的吸收和消除过程进行了跟踪研究。其中记录了农药的生物浓缩系数和消除动力学系数。此外，还用体内固相微萃取追踪了倍硫磷的代谢。该方法省时省力。在追踪过程中牺牲的实验动物要少得多。总的来说，这项研究为在实验室中廉价地测量生物浓缩系数以登记新出现的持久性有机污染物和检查可疑的持久性有机污染物提供了机会，并展示了体内固相微萃取在污染物毒代动力学研究中的潜在应用。
Bioconcentration factors (BCFs) measured in the laboratory are important for characterizing the bioaccumulative properties of chemicals entering the environment, especially the potential persistent organic pollutants (POPs), which can pose serious adverse effects on ecosystem and human health. Traditional lethal analysis methods are time-consuming and sacrifice too many experimental animals. In the present study, in vivo solid-phase microextraction (SPME) was introduced to trace the uptake and elimination processes of pesticides in living fish. BCFs and elimination kinetic coefficients of the pesticides were recorded therein. Moreover, the metabolism of fenthion was also traced with in vivo SPME. The method was time-efficient and laborsaving. Much fewer experimental animals were sacrificed during the tracing. In general, this study opened up an opportunity to measure BCFs cheaply in laboratories for the registering of emerging POPs and inspecting of suspected POPs, as well as demonstrated the potential application of in vivo SPME in the study of toxicokinetics of pollutants.