Baicalin regulates Treg/Th17 cell imbalance by inhibiting autophagy in allergic rhinitis
Baicalin regulates Treg/Th17 cell imbalance by inhibiting autophagy in allergic rhinitis
复制标题
黄芩苷通过抑制变应性鼻炎自噬调节Treg/Th17细胞失衡
DOI：
10.1016/j.molimm.2020.07.008
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发表时间：
2020-09-01
影响因子：
3.6
通讯作者：
Li, Yong
中科院分区：
文献类型：
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作者：
Li, Jing;Lin, Xiaoyan;Li, Yong
背景：黄芩苷具有抗氧化、抗过敏等多种生物学活性。本研究旨在探讨黄芩苷对变应性鼻炎（allergic rhinitis，AR）的治疗作用及其机制。采用实时荧光定量PCR、流式细胞术和Western blot检测鼻黏膜和外周血中Th 17和Treg细胞相关蛋白的表达水平。将小鼠随机分为对照组、卵清蛋白（OVA）组、L-黄芩苷组、H-黄芩苷组、DSGC组、3-MA组和H-黄芩苷+ Rapa组。采用Diff-Quik染色法检测小鼠变应性鼻炎的病情变化和嗜酸性粒细胞浸润情况，并进行评分;采用苏木精-伊红（H&E）染色和高碘酸盐-希夫（PAS）染色观察组织学变化。结果：临床病例分析发现AR患者存在Th 17/Treg失衡，自噬激活，而黄芩苷在体外可恢复Th 17/Treg平衡，抑制自噬。体内实验表明，黄芩苷抑制OVA诱导的过敏性鼻症状和自噬通路的激活，与3-MA的调节作用相同，而雷帕霉素可减弱H-黄芩苷的作用。黄芩苷还能减少鼻灌洗液中各种炎性细胞的浸润，防止上皮细胞的损伤，改善鼻粘膜厚度和粘液分泌。结论：黄芩苷对AR具有治疗作用，其作用机制可能是通过抑制自噬调节Th 17/Treg细胞分化来实现的。
Background: Baicalin has many biological properties such as anti-oxidation and anti-allergy. The current study aimed to explore the effect of Baicalin on allergic rhinitis (AR) and its potential mechanism of action.Methods: Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation. Expression levels of Th17 and Treg cells-related proteins in nasal mucosa and peripheral blood cells were detected by real-time quantitative PCR, flow cytometry and Western blot. The mice were randomly divided into Control, ovalbumin (OVA), L-Baicalin, H-Baicalin, DSGC, 3-MA, and H-Baicalin + Rapa groups. Changes of allergic rhinitis conditions and eosinophil infiltration of the mice were detected and scored by Diff-Quik staining, and histological changes were observed by Hematoxylin & Eosin (H&E) staining and Periodate Schiff (PAS) staining. Serological changes, expression levels of interleukin-17A (IL-17A), interleukin-10 (IL-10), eosinophilic cationic protein (ECP) and anti-OVA-specific antibodies were detected by Enzyme-linked immunosorbent assay (ELISA).Results: Clinical case analysis found that AR patients had a Th17/Treg imbalance and activated autophagy, however, Baicalin restored Th17/Treg cell balance and inhibited autophagy in vitro. in vivo experiments demonstrated that Baicalin inhibited OVA-induced allergic nasal symptoms and the activation of autophagy pathway, which was the same as the regulation of 3-MA, while Rapa could weaken the effects of H-baicalin. Moreover, Baicalin reduced the infiltration of different inflammatory cells of the nasal lavage fluid, prevented the damages to epithelial cells, and improved nasal mucosal thickness and mucus secretion. In addition, Baicalin regulated the balance of mouse anti-OVA-specific antibody levels and expressions of Th17/Treg-associated cytokines.Conclusion: Our study revealed that Baicalin can be used to treat AR, and the effect is realized through inhibiting autophagy to regulate Th17/Treg cell differentiation.