CHLOROGENIC ACID - AN ALLERGEN IN GREEN COFFEE BEAN
CHLOROGENIC ACID - AN ALLERGEN IN GREEN COFFEE BEAN
复制标题
DOI：
10.1038/192241a0
复制
发表时间：
1961-01-01
期刊：
影响因子：
64.8
通讯作者：
SEHON, AH
中科院分区：
文献类型：
--
作者：
FREEDMAN, SO;KRUPEY, J;SEHON, AH
自然1961年10月21日原酸产生阳性反应。1 pgm。的绿原酸足以在所有测试的受试者中诱导具有伪足的大风团和潮红反应。在被动转移测试与血清从这些工人中的五个，少至5 × 10-* pgm。在用0-05 ml.血清。用商业上获得的绿原酸和用Moores等人的方法从绿色咖啡的水提取物中分离的绿原酸发现了相同的结果。用绿原酸和绿色咖啡的水提取物进行交叉中和试验，以阐明这两种物质之间的过敏关系。在一系列试验中，正常人接受者皮肤的部位用从对绿色咖啡过敏的个体中获得的血清被动致敏。然后在24小时内通过皮内注射1 pgm对这些部位进行攻毒。绿原酸第二天，用100 pgm对相同部位进行再攻毒。绿色咖啡提取物。再激发时无反应，表明所有针对绿色咖啡的抗体先前已被绿原酸中和（表1）。
NATURE October 21, 1961 genic acid produced positive reactions. 1 pgm. of chlorogenic acid was sufficient to induce a large wheal and flare response with pseudopods in all the subjects tested. On passive transfer testing with the sera obtained from five of these workers, as little as 5 x 10-* pgm. of chlorogenic acid was required to elicit a reaction at a site sensitized with 0-05 ml. of serum. Identical results were found with chlorogenie acid obtained commercially and with chlorogenic acid isolated from the aqueous extract of green coffee by the method of Moores et al..*.Cross-neutralization tests were performed with chlorogenic acid and an aqueous extract of green coffee in order to elucidate the allergenic relationship between these two substances. In one series of tests, sites in the skin of normal human recipients were passively sensitized with sera obtained from indivi-duals allergic to green coffee. These sites were then challenged in 24 hr. by the intradermal injection of 1 pgm. of chlorogenic acid. On the following day, the same sites were re-challenged with 100 pgm. of green coffee extract. There were no reactions on re-challenge, indicating that all the antibody directed against the green coffee had been previously neutralized by the chlorogenic acid (Table 1).