Transcriptome analysis of Dlm mutants reveals the potential formation mechanism of lesion mimic in wheat
Transcriptome analysis of Dlm mutants reveals the potential formation mechanism of lesion mimic in wheat
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DOI：
10.1007/s10658-016-0975-x
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发表时间：
2016-12-01
影响因子：
1.8
通讯作者：
Li, Tao
中科院分区：
文献类型：
--
作者：
Li, Lei;Shi, Xuan;Li, Tao
以往的研究表明，中国小麦品种cv。Ning7840在成株期通过自发发生的过敏反应样表型（也称为病变模拟，LM）介导对锈病和白粉病具有高水平的广谱抗性。在这项研究中，两个EMS诱导的突变体的cv。宁7840、Dlm1和Dlm2表现为LM缺乏型。这两个突变体对白粉病感病。在抽穗期，当LM症状出现在宁7840叶片上时，从Dlm1、Dlm2和宁7840的剑叶中收集RNA，并进行测序，以揭示LM表型的分子机制。结果表明，二萜类化合物相关的胁迫反应作为一个间接调节LM表型，可能与宁7840的广谱抗性。氧化磷酸化、蛋白酶体和叶绿素参与了Dlm突变体中LM表达的抑制。这些研究结果为小麦对LM和广谱抗性的分子机制提供了新的认识。
Previous investigations demonstrated that Chinese wheat cv. Ning7840 confers a high level of broad-spectrum resistance to rusts and powdery mildew diseases at the adult plant stage mediated by a spontaneously occurring hypersensitive reaction-like phenotype (also called lesion mimic, LM). In this study, two EMS-induced mutants of cv. Ning7840, Dlm1 and Dlm2, showed the LM deficiency phenotype. These two mutants were susceptible to powdery mildew. At heading stage when the LM symptoms appear on the leaves of Ning7840, the RNAs were harvested from the flag leaves of Dlm1, Dlm2 and Ning7840 leaves and were sequenced to decipher the molecular mechanisms underlying LM phenotypes. The results suggest that the diterpenoid-related stress response acts as an indirect regulator of the LM phenotype and may be associated with broad-spectrum resistance in Ning7840. Oxidative phosphorylation, proteasome, and chlorophyll were involved in the suppression of the LM expression in the Dlm mutants. These results provide new insight into the molecular mechanism of LM and broad-spectrum resistance in wheat.