Optical intrinsic signal imaging with optogenetics reveals functional cortico-cortical connectivity at the columnar level in living macaques
Optical intrinsic signal imaging with optogenetics reveals functional cortico-cortical connectivity at the columnar level in living macaques
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DOI：
10.1038/s41598-019-42923-2
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发表时间：
2019-04
影响因子：
4.6
通讯作者：
Yu Nakamichi;Kai Okubo;Takayuki Sato;Mitsuhiro Hashimoto;M. Tanifuji
中科院分区：
文献类型：
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作者：
Yu Nakamichi;Kai Okubo;Takayuki Sato;Mitsuhiro Hashimoto;M. Tanifuji
尽管对灵长类的认知功能进行了广泛的研究，但了解神经回路水平的解剖连接如何与不同皮质区域的信息传输有关仍然是原始的。需要新的技术来可视化活着的猴子的区域间解剖连接，并将其直接与神经生理功能联系起来。在这里，我们开发了一种新的方法来研究这种结构-功能关系，通过结合光学内在信号成像(OISI)和活猴子的光遗传刺激(OTO-OISI)。该方法包括在一个区域(源)表达ChannelRhodophsin-2，然后在另一个区域(靶)对光遗传激活进行光学成像。我们成功地用V1/V2边界区域之间的半球间柱状投影图案展示了该方法的潜力。与光遗传学和功能磁共振成像(opto-fMRI)的结合不同，opto-OISI的优势是使我们能够检测到小簇神经元的反应，即使这些簇是稀疏分布的。我们认为，光学OISI是在神经回路水平上理解认知功能的一种强有力的方法，它直接将区域间回路与精细的结构和功能联系起来。
Despite extensive research on primate cognitive function, understanding how anatomical connectivity at a neural circuit level relates to information transformation across different cortical areas remains primitive. New technology is needed to visualize inter-areal anatomical connectivity in living monkeys and to tie this directly to neurophysiological function. Here, we developed a novel method to investigate this structure-function relationship, by combining optical intrinsic signal imaging (OISI) with optogenetic stimulation in living monkeys (opto-OISI). The method involves expressing channelrhodophsin-2 in one area (source) followed by optical imaging of optogenetic activations in the other area (target). We successfully demonstrated the potential of the method with interhemispheric columnar projection patterns between V1/V2 border regions. Unlike the combination of optogenetics and functional magnetic resonance imaging (opto-fMRI), opto-OISI has the advantage of enabling us to detect responses of small clusters of neurons, even if the clusters are sparsely distributed. We suggest that opto-OISI can be a powerful approach to understanding cognitive function at the neural circuit level, directly linking inter-areal circuitry to fine-scale structure and function.