Comprehensive application of an mtDsRed2-Tg mouse strain for mitochondrial imaging
Comprehensive application of an mtDsRed2-Tg mouse strain for mitochondrial imaging
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mtDsRed2-Tg 小鼠品系在线粒体成像中的综合应用
DOI：
10.1007/s11248-011-9539-1
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发表时间：
2012
影响因子：
3
通讯作者：
H
中科院分区：
文献类型：
--
作者：
Yamaguchi;J.;Nishiyama;S.;Shimanuki;M.;Ono;T.;Sato;A.;Nakada;K.;Hayashi;J-I.;Yonekawa;H.;Shitara;H
线粒体是许多细胞功能所必需的，如氧化磷酸化和钙稳态；因此，线粒体功能障碍可导致许多疾病，包括神经疾病。最近，线粒体的动力学，如融合、分裂和运输，已经通过时间推移成像系统在活细胞中被可视化。线粒体形态的变化可作为评价线粒体生物学功能活性的指标。在这里，我们报告了一个转基因小鼠品系mtDsRed2-TG，它只在线粒体中表达红色荧光蛋白DsRed2。共聚焦显微镜下可清楚地观察到该菌株不同组织中线粒体的形态。近年来，许多表达增强型绿色荧光蛋白(EGFP)标记蛋白的转基因小鼠品系已被建立用于体内生理分析。这些菌株与mtDsRed2-TG小鼠交配后，利用荧光成像系统同时检测到红色的线粒体和绿色的蛋白质，并可以在细胞和组织中对线粒体和这些蛋白质的相互作用进行形态分析。因此，mtDsRed2-Tg小鼠可以成为线粒体功能生物成像研究的有力工具。
Mitochondria are essential for many cellular functions such as oxidative phosphorylation and calcium homeostasis; consequently, mitochondrial dysfunction could cause many diseases, including neurological disorders. Recently, mitochondrial dynamics, such as fusion, fission, and transportation, have been visualized in living cells by using time-lapse imaging systems. The changes in mitochondrial morphology could be an indicator for estimating the activity of mitochondrial biological function. Here, we report a transgenic mouse strain, mtDsRed2-Tg, which expresses a red fluorescent protein, DsRed2, exclusively in mitochondria. Mitochondrial morphology could be clearly observed in various tissues of this strain under confocal microscope. Recently, many transgenic mouse strains in which enhanced green fluorescent protein (EGFP)-tagged proteins of interest are expressed have been established for physiological analysis in vivo. After mating these strains with mtDsRed2-Tg mice, red-colored mitochondria and green-colored proteins were detected simultaneously using fluorescent imaging systems, and the interactions between mitochondria and those proteins could be morphologically analyzed in cells and tissues of the F1hybrids. Thus, mtDsRed2-Tg mice can be a powerful tool for bioimaging studies on mitochondrial functions.