EGF signaling patterns the feather array by promoting the interbud fate
EGF signaling patterns the feather array by promoting the interbud fate
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DOI：
10.1016/s1534-5807(03)00021-2
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发表时间：
2003-02-01
影响因子：
11.8
通讯作者：
Niswander, L
中科院分区：
文献类型：
--
作者：
Atit, R;Conlon, R;Niswander, L
羽芽依次形成六角形阵列。来自羽芽的骨形态发生蛋白（BMP）信号抑制邻近芽间组织的芽形成，但BMP或其他因素是否积极促进芽间命运和图案尚不清楚。我们发现，表皮生长因子（EGF）信号传导积极的行为，以建立interbud身份。EGF和活性EGF受体（EGFR）在芽间区域表达。外源性EGF刺激表皮增殖和扩大interbud基因表达，与羽芽基因表达和形态的同时损失。相反，EGFR抑制剂导致芽间命运的丧失和羽芽命运的获得增加。EGF信号直接作用于表皮，并且独立于BMP信号。解释互芽促进信号的能力的时间发生在比以前预期的更早的发育阶段。这些数据表明EGFR信号传导积极促进芽间身份。
Feather buds form sequentially in a hexagonal array. Bone morphogenetic protein (BMP) signaling from the feather bud inhibits bud formation in the adjacent interbud tissue, but whether interbud fate and patterning is actively promoted by BMP or other factors is unclear. We show that epidermal growth factor (EGF) signaling acts positively to establish interbud identity. EGF and the active EGF receptor (EGFR) are expressed in the interbud regions. Exogenous EGF stimulates epidermal proliferation and expands interbud gene expression, with a concurrent loss of feather bud gene expression and morphology. Conversely, EGFR inhibitors result in the loss of interbud fate and increased acquisition of feather bud fate. EGF signaling acts directly on the epidermis and is independent of BMP signaling. The timing of competence to interpret interbud-promoting signals occurs at an earlier developmental stage than previously anticipated. These data demonstrate that EGFR signaling actively promotes interbud identity.