Genome-Wide QTL Mapping for Wheat Processing Quality Parameters in a Gaocheng 8901/Zhoumai 16 Recombinant Inbred Line Population.
Genome-Wide QTL Mapping for Wheat Processing Quality Parameters in a Gaocheng 8901/Zhoumai 16 Recombinant Inbred Line Population.
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藁城8901/周麦16重组自交系群体小麦加工品质参数的全基因组QTL定位
DOI：
10.3389/fpls.2016.01032
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发表时间：
2016
影响因子：
5.6
通讯作者：
He Z
中科院分区：
文献类型：
--
作者：
Jin H;Wen W;Liu J;Zhai S;Zhang Y;Yan J;Liu Z;Xia X;He Z
面团的流变学特性和淀粉糊化特性是决定面包小麦加工品质的重要因素。本研究采用小麦90和660K单核苷酸多态(SNP)芯片技术，利用小麦90K和660K单核苷酸多态(SNP)芯片技术，利用小麦90和660K单核苷酸多态(SNP)芯片技术，对高城8901/周麦16的重组自交系(RIL)群体进行了面团流变学和淀粉糊化特性的QTL定位。用小麦90和660K SNP构建的46,961个SNP标记构建的高密度连锁图谱全长4121 cM，平均染色体长度为196.2 cM，标记密度为0.09 cM/标记，其中6596个新的SNP标记被锚定在面包小麦连锁图上，其中1046个和5550个标记分别来自90和660K SNP分析。复合区间作图在除4D外的20条染色体上检测到119个加性QTL，其中15个QTL解释了两个或三个环境中表型变异的10%以上。其中12个为新的QTL，17个为RVA参数，55个为Mixolab参数。共检测到11个QTL簇。这些紧密连锁的SNP标记可用于分子标记辅助小麦育种，并与Kasp技术相结合，以改良面包小麦的加工品质。
Dough rheological and starch pasting properties play an important role in determining processing quality in bread wheat (Triticum aestivum L.). In the present study, a recombinant inbred line (RIL) population derived from a Gaocheng 8901/Zhoumai 16 cross grown in three environments was used to identify quantitative trait loci (QTLs) for dough rheological and starch pasting properties evaluated by Mixograph, Rapid Visco-Analyzer (RVA), and Mixolab parameters using the wheat 90 and 660 K single nucleotide polymorphism (SNP) chip assays. A high-density linkage map constructed with 46,961 polymorphic SNP markers from the wheat 90 and 660 K SNP assays spanned a total length of 4121 cM, with an average chromosome length of 196.2 cM and marker density of 0.09 cM/marker; 6596 new SNP markers were anchored to the bread wheat linkage map, with 1046 and 5550 markers from the 90 and 660 K SNP assays, respectively. Composite interval mapping identified 119 additive QTLs on 20 chromosomes except 4D; among them, 15 accounted for more than 10% of the phenotypic variation across two or three environments. Twelve QTLs for Mixograph parameters, 17 for RVA parameters and 55 for Mixolab parameters were new. Eleven QTL clusters were identified. The closely linked SNP markers can be used in marker-assisted wheat breeding in combination with the Kompetitive Allele Specific PCR (KASP) technique for improvement of processing quality in bread wheat.