Inter- and intralineage recombinants are common in natural populations of Turnip mosaic virus
Inter- and intralineage recombinants are common in natural populations of Turnip mosaic virus
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DOI：
10.1099/vir.0.80124-0
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发表时间：
2004-09-01
影响因子：
3.8
通讯作者：
Ohshima, K
中科院分区：
文献类型：
--
作者：
Tan, ZY;Wada, Y;Ohshima, K
芜菁花叶病毒（TuMV）的基因组重组图组装使用的数据从19个完整的基因组序列，以前报道，和一个复合样品的基因组的三个区域，三分之一的总，一个代表性的亚洲范围内收集的70个分离物。因此，共分析了89个世界各地来源的分离株的重组体。发现18个重组位点分布在整个基因组的5'三分之二处，但在3'三分之一处只有两个;因此，所检查的P1和Nla-VPg基因序列中有24%和35%是重组体，而相应的Nla-Pro和CP基因序列中只有1%是重组体。发现了双亲来自同一谱系或不同谱系的重组子，并发现了一些具有特定谱系特征的重组位点。如前所述，大多数菌株BR重组体属于亚洲-BR组，并且可以得出结论，该谱系来自最近的迁移，而许多来自亚洲的菌株B重组体属于世界-B组。同样，该组中大部分分离株是重组体。一些重组位点仅在特定谱系中发现，因此似乎更可能是来自单个重组事件的存活后代，而不是在重组热点处发生的多个事件的后代。看来，重组位点的存在，以及序列的相似性，可以用来跟踪TuMV的迁移和进化。
A recombination map of the genome of Turnip mosaic virus (TuMV) was assembled using data from 19 complete genomic sequences, previously reported, and a composite sample of three regions of the genome, one-third in total, of a representative Asia-wide collection of 70 isolates. Thus, a total of 89 isolates of worldwide origin was analysed for recombinants. Eighteen recombination sites were found spaced throughout the 5' two-thirds of the genome, but there were only two in the 3' one-third; thus, 24 and 35 % of the P1 and Nla-VPg gene sequences examined were recombinants, whereas only 1 % of the corresponding Nla-Pro and CP gene sequences were recombinants. Recombinants with parents from the same orfrom different lineages were found, and some recombination sites characterized particular lineages. Most of the strain BR recombinants belonged to the Asian-BR group, as defined previously, and it was concluded that this lineage resulted from a recent migration, whereas many of the strain B recombinants from Asia fell into the world-B group. Again, a large proportion of isolates in this group were recombinants. Some recombination sites were found only in particular lineages, and hence seemed more likely to be the surviving progeny from single recombinational events, rather than the progeny of multiple events occurring at recombination hotspots. It seems that the presence of recombination sites, as well as sequence similarities, may be used to trace the migration and evolution of TuMV.