Interleukin-1 beta stimulates colony-stimulating factor-1 production in placental villous core mesenchymal cells.
Interleukin-1 beta stimulates colony-stimulating factor-1 production in placental villous core mesenchymal cells.
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Interleukin-1 beta 刺激胎盘绒毛核心间充质细胞产生集落刺激因子 1。
DOI：
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发表时间：
1992
影响因子：
5.8
通讯作者：
S. Kauma
中科院分区：
文献类型：
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作者：
J. Harty;S. Kauma
已知人胎盘可产生造血生长因子，包括集落刺激因子-1 （CSF-1）。我们之前已经证明了妊娠期间脱个体化子宫内膜产生白细胞介素-1 β (IL-1 β)。由于IL-1刺激成纤维细胞、内皮细胞和骨髓基质细胞中CSF-1的产生，我们本研究旨在确定IL-1是否也能调节胎盘绒毛核间充质细胞中CSF-1的产生。利用酶切分离滋养细胞和绒毛核的初步研究表明，CSF-1 mRNA主要在胎盘绒毛核中表达。随后，从妊娠9 ~ 16周的胎盘中分离出完整的胎盘绒毛，酶解后建立绒毛核间充质细胞的长期单层培养，研究绒毛核细胞CSF-1产生的调控。细胞形态和免疫组化检测波形蛋白、细胞角蛋白、CD45抗原证实培养细胞95%以上为间充质成纤维细胞。时间过程实验表明，在IL-1 β (10 ng/mL)处理后3小时，CSF-1 mRNA表达量最大增加约为基线水平的6.0倍，而在IL-1 β处理后3小时，培养基中首次检测到CSF-1蛋白产量增加，并在42小时内逐渐增加。绒毛核间充质细胞与0-10 ng/mL IL-1 β孵育显示出CSF-1 mRNA表达和蛋白质产生的特定剂量-反应关系，首先在0.10 ng/mL时出现，在10 ng/mL IL-1 β时达到最大。这些结果表明，IL-1 β可以在体外刺激胎盘绒毛核间充质细胞产生CSF-1，并提示蜕膜IL-1可能调节体内胎盘CSF-1的产生。
The human placenta is known to produce hematopoietic growth factors, including colony-stimulating factor-1 (CSF-1). We have previously demonstrated interleukin-1 beta (IL-1 beta) production by decidualized endometrium during pregnancy. Since IL-1 stimulates CSF-1 production in fibroblasts, endothelial cells, and bone marrow stromal cells, our present study was designed to determine whether IL-1 could also regulate CSF-1 production by placental villous core mesenchymal cells. Initial studies using enzymatic digestion separation of the trophoblast and villous core demonstrated that CSF-1 mRNA is mainly expressed in the placental villous core. Subsequently, long term monolayer cultures of villous core mesenchymal cells isolated from 9- to 16-week gestation placentas were established after enzymatic dissociation of intact placental villi to study the regulation of villous core cell CSF-1 production. The morphology of the cells and immunohistochemical staining for vimentin, cytokeratin, and CD45 antigen confirmed that cultured cells were more than 95% mesenchymal fibroblasts. Time-course experiments demonstrated a maximal increase in CSF-1 mRNA expression of approximately 6.0-fold over baseline levels 3 h after IL-1 beta treatment (10 ng/mL), whereas increased CSF-1 protein production was first detected in the culture medium 3 h after IL-1 beta treatment and rose progressively over 42 hours. Villous core mesenchymal cells incubated with 0-10 ng/mL IL-1 beta demonstrated a specific dose-response relationship for CSF-1 mRNA expression and protein production, first seen at 0.10 ng/mL and maximal with 10 ng/mL IL-1 beta. These results demonstrate that production of CSF-1 by placental villous core mesenchymal cells can be stimulated by IL-1 beta in vitro and suggest that decidual IL-1 may regulate placental CSF-1 production in vivo.