Disruption of MoCMK1, encoding a putative calcium/calmodulin-dependent kinase, in Magnaporthe oryzae
Disruption of MoCMK1, encoding a putative calcium/calmodulin-dependent kinase, in Magnaporthe oryzae
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米霉中 MoCMK1 的破坏，编码一种推定的钙/钙调蛋白依赖性激酶
DOI：
10.1016/j.micres.2009.08.007
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发表时间：
2010-01-01
影响因子：
6.7
通讯作者：
Lin, Fu-Cheng
中科院分区：
文献类型：
--
作者：
Liu, Xiao-Hong;Lu, Jian-Ping;Lin, Fu-Cheng
Ca ~（2+）是植物和动物体内传递外部信号和激活细胞过程的第二信使。Ca 2+介导的信号转导参与了真核细胞中多种基本生理过程的关键途径。然而，对丝状真菌中Ca 2+介导的信号转导的分子机制知之甚少。在这项研究中，MoCMK 1基因，编码一个假定的Ca 2 +/钙调素依赖性激酶，被确定在稻瘟病菌Magnaporthe magnesium。通过靶向基因置换获得了3个MoCMK 1缺失突变体。MoCMK 1突变体的菌落具有稀疏的气生菌丝和较少的分生孢子比野生型菌株在完全培养基上。三角洲Mocmk 1突变体的孢子萌发和附着胞形成延迟。在喷雾接种试验中，与野生型菌株Guy 11相比，Delta Mocmk 1突变体表现出较弱的感染感病水稻品种CO-39的能力。这些结果表明MoCMK 1在稻瘟病菌的致病性中起关键作用。(C)2009年Elsevier GmbH。All rights reserved.
Ca2+ is a second messenger in pathways that transduce external signals and activate cellular processes in plants and animals. Ca2+-mediated signal transduction is involved in key pathways that contribute to a variety of fundamental physiological processes in eukaryotic cells. However, little is known about the molecular mechanisms of Ca2+ -mediated signal transduction in filamentous fungi. In this study, the MoCMK1 gene, encoding a putative Ca2+/calmodulin-dependent kinase, was identified in the rice blast fungus Magnaporthe oryzae. Three MoCMK1 deletion mutants were obtained by a targeted gene replacement. Colonies of the MoCMK1 mutants had sparse aerial hyphae and fewer conidia than the wild-type strain on complete medium. Conidial germination and appressorial formation were delayed in the Delta Mocmk1 mutants. In spray inoculation tests, Delta Mocmk1 mutants exhibited a weakened ability to infect the susceptible rice cultivar CO-39, compared to the wildtype strain Guy11. These results showed that MoCMK1 plays key roles in the pathogenicity of the rice blast fungus. (C) 2009 Elsevier GmbH. All rights reserved.