In vivo blockade of acetylcholinesterase increases intraovarian acetylcholine and enhances follicular development and fertility in the rat
In vivo blockade of acetylcholinesterase increases intraovarian acetylcholine and enhances follicular development and fertility in the rat
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DOI：
10.1038/srep30129
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发表时间：
2016-07-21
影响因子：
4.6
通讯作者：
Lara, Hernan E.
中科院分区：
文献类型：
--
作者：
Urra, Javier;Blohberger, Jan;Lara, Hernan E.
卵泡的生长和分化受全身和局部因素的调节，其中可能包括乙酰胆碱(ACh)。生长中的卵泡和黄体细胞的颗粒细胞产生ACh，在培养的GCs中，它通过M受体发挥营养作用。然而，这种作用并没有在体内进行研究。在确定大鼠卵巢GC和黄体细胞表达ACh代谢酶(AChE)后，我们观察了局部应用AChE抑制剂石杉碱甲(HupA)的结果。对照组用生理盐水。局部注射HupA 4周后，卵巢ACh含量增加。发情周期没有改变，这表明HupA的作用范围是局部受限的。HupA组的原始卵泡和初级卵泡数量未受影响，但较小的次级卵泡数量显著增加。此外，黄体数量的显著增加表明排卵事件增加。在支持方面，如交配时所示，HupA处理的雌性显著增加了着床部位和更多的幼崽。因此，这些数据支持ACh在卵泡发育和排卵中的营养作用，并指出ACh在女性生育中的重要作用。
Growth and differentiation of ovarian follicles are regulated by systemic and local factors, which may include acetylcholine (ACh). Granulosa cells (GCs) of growing follicles and luteal cells produce ACh and in cultured GCs it exerts trophic actions via muscarinic receptors. However, such actions were not studied in vivo. After having established that rat ovarian GCs and luteal cells express the ACh-metabolizing enzyme ACh esterase (AChE), we examined the consequences of local application of an AChE inhibitor, huperzine A (HupA), by osmotic minipump delivery into the ovarian bursa of hemiovariectomized rats. Saline was used in the control group. Local delivery of HupA for 4 weeks increased ovarian ACh content. Estrus cyclicity was not changed indicating a locally restricted range of HupA action. The number of primordial and primary follicles was unaffected, but small secondary follicles significantly increased in the HupA group. Furthermore, a significant increase in the number of corpora lutea suggested increased ovulatory events. In support, as shown upon mating, HupA-treated females had significantly increased implantation sites and more pups. Thus the data are in support of a trophic role of ACh in follicular development and ovulation and point to an important role of ACh in female fertility.