IDENTIFICATION AND ARRANGEMENT OF THE DNA-SEQUENCE RECOGNIZED IN SPECIFIC TRANSFORMATION OF NEISSERIA-GONORRHOEAE
IDENTIFICATION AND ARRANGEMENT OF THE DNA-SEQUENCE RECOGNIZED IN SPECIFIC TRANSFORMATION OF NEISSERIA-GONORRHOEAE
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淋病奈瑟菌特异性转化中识别的 DNA 序列的鉴定和排列
DOI：
10.1073/pnas.85.18.6982
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发表时间：
1988-09-01
影响因子：
11.1
通讯作者：
SCOCCA, JJ
中科院分区：
文献类型：
--
作者：
GOODMAN, SD;SCOCCA, JJ
从淋病奈瑟氏菌的DNA片段，克隆和繁殖的土方ichis大肠杆菌，竞争性抑制淋球菌转化的能力进行了测试。测定并比较了活性片段的核苷酸序列，这些序列共同含有序列5'' GCCGTCTGAA 3''。亚克隆研究证实该序列为淋球菌DNA识别位点。从N.淋病DNA在其反向重复序列的紧邻处含有该序列。因为序列的单个拷贝起识别位点的作用，所以特异性结合不需要反向重复。染色体识别序列的二分体对称排列可以形成稳定的茎环结构，其可以充当转录的终止子或衰减子。这些反向重复序列位于长开放阅读框的边界处。识别序列也构成淋球菌基因的另外两个可能终止子的一部分。我们的结论是淋球菌的转化DNA的识别信号是一个常见的转录终止子序列的组成部分。这种调节功能可能解释了革兰氏阴性可转化细菌染色体中识别序列的起源和维持。
DNA segments from Neisseria gonorrhoeae, cloned and propagated in Escherichis coli, were tested for the ability to competitively inhibited gonococcal transformation. The nucleotide sequences of active segments were determined and compared; these sequences contained the sequence 5'' GCCGTCTGAA 3'' in common. Subcloning studies confirmed identity of this sequence as the gonococcal DNA recognition site. The three instances of the recognition sequence isolated from N. gonorrhoeae DNA contain the sequence in the immediate neighborhood of its inverted repeat. Because a single copy of the sequence functions as a recognition site, the inverted duplication is not required for specific binding. The dyad symmetric arrangements of the chromosomal recognition sequences may form stable stem-loop structures that can function as terminators or attenuators of transcription. These inverted repeats are located at the boundaries of long open reading frames. The recognition sequence also constitutes part of two other probable terminators of gonococcal genes. We conclude that the signal for recognition of transforming DNA by gonococci is a frequent component of transcriptional terminator sequences. This regulatory function might account for the origin and maintenance of recognition sequences in the chromosomes of Gram-negative transformable bacteria.