Aminomalonic acid: identification in Escherichia coli and atherosclerotic plaque.
Aminomalonic acid: identification in Escherichia coli and atherosclerotic plaque.
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DOI：
10.1073/pnas.81.3.722
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发表时间：
1984-02
影响因子：
11.1
通讯作者：
J. J. Buskirk-J.;Wolff M. Kirsch;D. L. Kleyer;Robert M. Barkley;Tad H. Koch
中科院分区：
文献类型：
--
作者：
J. J. Buskirk-J.;Wolff M. Kirsch;D. L. Kleyer;Robert M. Barkley;Tad H. Koch
氨基丙二酸（Ama）是从大肠杆菌和人动脉粥样硬化斑块中分离得到的。Ama的存在具有重要的生物学意义，因为丙二酸部分可能赋予蛋白质钙结合特性。通过厌氧碱性水解获得AMA，并通过色谱行为、定量酸介导脱羧生成甘氨酸和明确的气相色谱/质谱检测进行鉴定。天然Ama的色谱、化学和质谱特性与合成化合物相同。氨基酸分析和GC/质谱分析也揭示了β-羧基天冬氨酸和γ-羧基谷氨酸在人动脉粥样硬化斑块的碱性水解产物中的存在。Ama与β-羧基天冬氨酸与γ-羧基谷氨酸的比例为20：1：10，每1，000个甘氨酸残基中Ama的量为0.2。Ama是一种相对不稳定的次要氨基酸，存在于细菌或组织等复杂结构中。这也许可以解释为什么尽管进行了深入的调查，但它以前一直没有被发现。
Aminomalonic acid (Ama) has been isolated from proteins of Escherichia coli and human atherosclerotic plaque. The presence of Ama has important biological implications because the malonic acid moiety potentially imparts calcium binding properties to protein. Ama was obtained by anaerobic alkaline hydrolysis and identified by chromatographic behavior, quantitative acid-mediated decarboxylation to glycine, and unambiguous gas chromatographic/mass spectral detection. The chromatographic, chemical, and mass spectral properties of naturally occurring Ama were identical to those of the synthetic compound. Amino acid analysis and GC/mass spectrometry also revealed the presence of beta-carboxyaspartic acid and gamma-carboxyglutamic acid in the base hydrolysate of human atherosclerotic plaque. The ratio of Ama to beta-carboxyaspartic acid to gamma-carboxyglutamic acid was 20:1:10, and the quantity of Ama per 1,000 glycine residues was 0.2. Ama is a relatively unstable, minor amino acid in complex structures such as bacteria or tissues. This may explain why it has escaped detection previously, despite intensive investigation.